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Titolo:
Tandem repeat of a transcriptional enhancer upstream of the sterol 14 alpha-demethylase gene (CYP51) in Penicillium digitatum
Autore:
Hamamoto, H; Hasegawa, K; Nakaune, R; Lee, YJ; Makizumi, Y; Akutsu, K; Hibi, T;
Indirizzi:
Univ Tokyo, Dept Agr & Environm Biol, Plant Pathol Lab, Bunkyo Ku, Tokyo 1138657, Japan Univ Tokyo Tokyo Japan 1138657 thol Lab, Bunkyo Ku, Tokyo 1138657, Japan Biooriented Technol Res Advancement Inst, BRAIN, Ohmiya, Saitama 3310044, Japan Biooriented Technol Res Advancement Inst Ohmiya Saitama Japan 3310044 pan Natl Inst Fruit Tree Sci, Akitsu, Hiroshima 7292494, Japan Natl Inst FruitTree Sci Akitsu Hiroshima Japan 7292494 ma 7292494, Japan Ibaraki Univ, Fac Agr, Ami, Ibaraki 3000393, Japan Ibaraki Univ Ami Ibaraki Japan 3000393 c Agr, Ami, Ibaraki 3000393, Japan
Titolo Testata:
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
fascicolo: 8, volume: 66, anno: 2000,
pagine: 3421 - 3426
SICI:
0099-2240(200008)66:8<3421:TROATE>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
AZOLE ANTIFUNGAL AGENTS; RESISTANT CANDIDA-ALBICANS; MULTIDRUG-RESISTANCE; LANOSTEROL 14-ALPHA-DEMETHYLASE; FLUCONAZOLE RESISTANCE; 14-DEMETHYLASE P450; FUNGUS; DEMETHYLASE; FUNGICIDES; CONTRIBUTE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Hamamoto, H Univ Tokyo, Dept Agr & Environm Biol, Plant Pathol Lab, BunkyoKu, Tokyo 1138657, Japan Univ Tokyo Tokyo Japan 1138657 nkyo Ku, Tokyo 1138657, Japan
Citazione:
H. Hamamoto et al., "Tandem repeat of a transcriptional enhancer upstream of the sterol 14 alpha-demethylase gene (CYP51) in Penicillium digitatum", APPL ENVIR, 66(8), 2000, pp. 3421-3426

Abstract

We investigated the mechanism of resistance to demethylation inhibitors (DMI) in Penicillium digitatum by isolating the CYP51 gene, which encodes thetarget enzyme (P450(14DM)) of DMI, from three DMI-resistant and three DMI-sensitive strains. The structural genes of all six strains were identical, but in the promoter region, a unique 126-bp sequence was tandemly repeated five times in the DMI-resistant strains and was present only once in the DMI-sensitive strains. Constitutive expression of CYP51 in the resistant strains was about 100-fold higher than that in the sensitive strains. We introduced CYP51, including the promoter region, from a DMI-resistant strain intoa DMI-sensitive strain, which rendered the: transformants DMI resistant and increased CYP51 expression. We also found that if the number of copies ofthe repeat was reduced to two, resistance and CYP51 expression also decreased, These results indicate that the 126-bp unit acts as a transcriptional enhancer and that a tandem repeat of the unit enhances CYP51 expression, resulting in DR;II resistance. This is a new fungicide resistance mechanism for filamentous fungi.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/07/20 alle ore 21:29:27