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Titolo:
Micellar liquid chromatographic separation of amino acids using pre- and post-column o-phthalaldehyde/N-acetylcysteine derivatization
Autore:
Lopez-Grio, S; Torres-Lapasio, JR; Baeza-Baeza, JJ; Garcia-Alvarez-Coque, MC;
Indirizzi:
Univ Valencia, Fac Quim, Dept Quim Analit, E-46100 Valencia, Spain Univ Valencia Valencia Spain E-46100 uim Analit, E-46100 Valencia, Spain Univ Navarra, Fac Ciencias, Dept Quim & Edafol, E-31080 Pamplona, Spain Univ Navarra Pamplona Spain E-31080 im & Edafol, E-31080 Pamplona, Spain
Titolo Testata:
ANALYTICA CHIMICA ACTA
fascicolo: 2, volume: 418, anno: 2000,
pagine: 153 - 165
SICI:
0003-2670(20000809)418:2<153:MLCSOA>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
PHASE; DERIVATIVES; PEPTIDES;
Keywords:
primary proteic amino acids; o-phthalaldehyde; N-acetyl-L-cysteine; pre- and post-column derivatization; micellar eluents;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Physical, Chemical & Earth Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Garcia-Alvarez-Coque, MC Univ Valencia, Fac Quim, Dept Quim Analit, E-46100 Valencia, Spain Univ Valencia Valencia Spain E-46100 cia, Spain
Citazione:
S. Lopez-Grio et al., "Micellar liquid chromatographic separation of amino acids using pre- and post-column o-phthalaldehyde/N-acetylcysteine derivatization", ANALYT CHIM, 418(2), 2000, pp. 153-165

Abstract

The isocratic analysis of primary proteic amino acids using a C-18 column,micellar mobile phases of sodium dodecyl sulphate (SDS) and 1-propanol at varying pH, and pre- and po st-column formation of the o-phthalaldehyde (OPA)/N-acetyl-L-cysteine (NAC) isoindoles, is examined. An interpretive optimization strategy was applied to find the best separation conditions. For this purpose, empirical polynomial models were used to describe the elution behavior of the isoindoles and free amino acids, using as separation factorsthe concentrations of surfactant and modifier (two-factor problem), or these two factors and the pH of the mobile phase (three-factor problem). The separation of the OPA/NAC amino acid isoindoles was very poor: only eight compounds could be separated satisfactorily. In contrast, 14 free amino acids(ordered according to their elution times: aspartic acid, glutamine, threonine, cysteine, alanine, tyrosine, valine, methionine, phenylalanine, leucine, lysine, histidine, tryptophan and arginine) could be well resolved with0.05 M SDS-1.2% propanol at pH 3 in 42 min, partially due to the higher efficiencies frequently five- to 10-fold those achieved for the isoindoles atthis pH. A mobile phase of 0.05 M SDS without alcohol allowed the resolution of a mixture of aspartic acid, glutamine, asparagine, glutamic acid, threonine, cysteine and alanine, which eluted at the lower retention times. Inthese conditions, only serine and glycine overlapped with glutamine and threonine, respectively. (C) 2000 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 07:54:52