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Titolo:
HPLC analysis of tricyclic antidepressants in skin diffusion studies
Autore:
Gandhi, R; Sharma, P; Chawla, HPS; Kaul, CL; Panchagnula, R;
Indirizzi:
Natl Inst Pharmaceut Educ & Res, Dept Pharmaceut, SAS Nagar 160062, Punjab, India Natl Inst Pharmaceut Educ & Res SAS Nagar Punjab India 160062 njab, India Natl Inst Pharmaceut Educ & Res, Dept Pharmaceut Technol, SAS Nagar 160062, Punjab, India Natl Inst Pharmaceut Educ & Res SAS Nagar Punjab India 160062 njab, India
Titolo Testata:
STP PHARMA SCIENCES
fascicolo: 3, volume: 10, anno: 2000,
pagine: 234 - 238
SICI:
1157-1489(200005/06)10:3<234:HAOTAI>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Keywords:
HPLC analysis; tricyclic antidepressants; amitriptyline HCl; skin diffusion; radiochemical analysis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
7
Recensione:
Indirizzi per estratti:
Indirizzo: Panchagnula, R Natl Inst Pharmaceut Educ & Res, Dept Pharmaceut, Sector 67, SAS Nagar 160062, Punjab, India Natl Inst Pharmaceut Educ & Res Sector 67SAS Nagar Punjab India 160062
Citazione:
R. Gandhi et al., "HPLC analysis of tricyclic antidepressants in skin diffusion studies", STP PHARM S, 10(3), 2000, pp. 234-238

Abstract

The aim of this study was to develop and validate a high-performance liquid chromatography method for the determination of tricyclic antidepressants in skin permeation studies. The chromatographic system involved the use of a Shim-pack (C-18) column with pH 3.0 sodium phosphate buffer-acetonitrile as the mobile phase; detection was set at 238 and 250 nm for amitriptyline HCl and imipramine HCl, respectively. Calibration curves of amitriptyline HCl and imipramine HCl were obtained in three different media namely, methanol, pH 7.4 phosphate buffer and physiological saline. Skin permeation studies were peformed using Sprague-Dawley rat skin and drug samples were analysed by the HPLC method. this technique was compared with radiochemical analysis using H-3 imipramine. The HPLC method proposed was successfully applicable to the continuous monitoring of tricyclic antidepressants in skin permeation studies, and the results were comparable to the radiochemical method in the case of imipramine HCl.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 06:48:50