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Titolo:
Cell-type-specific calcium responses to drought, salt and cold in the Arabidopsis root
Autore:
Kiegle, E; Moore, CA; Haseloff, J; Tester, MA; Knight, MR;
Indirizzi:
Univ Cambridge, Dept Plant Sci, Cambridge CB2 3EA, England Univ CambridgeCambridge England CB2 3EA Sci, Cambridge CB2 3EA, England Univ Oxford, Dept Plant Sci, Oxford OX1 3RB, England Univ Oxford Oxford England OX1 3RB pt Plant Sci, Oxford OX1 3RB, England
Titolo Testata:
PLANT JOURNAL
fascicolo: 2, volume: 23, anno: 2000,
pagine: 267 - 278
SICI:
0960-7412(200007)23:2<267:CCRTDS>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
TOBACCO SUSPENSION-CULTURE; GREEN FLUORESCENT PROTEIN; CYTOSOLIC-FREE CALCIUM; STOMATAL GUARD-CELLS; ABSCISIC-ACID; CYTOPLASMIC CALCIUM; SIGNAL-TRANSDUCTION; GENE-EXPRESSION; FREE CA2+; PLANTS;
Keywords:
calcium; GAL4; GFP; endodermis; stress; aequorin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
53
Recensione:
Indirizzi per estratti:
Indirizzo: Kiegle, E Ceres Inc, 3007 Malibu Canyon Rd, Malibu, CA 90265 USA Ceres Inc3007 Malibu Canyon Rd Malibu CA USA 90265 CA 90265 USA
Citazione:
E. Kiegle et al., "Cell-type-specific calcium responses to drought, salt and cold in the Arabidopsis root", PLANT J, 23(2), 2000, pp. 267-278

Abstract

Little is known about the signalling processes involved in the response ofroots to abiotic stresses. The Arabidopsis root is a model system of root anatomy with a simple architecture and is amenable to genetic manipulation. Although it is known that the root responds to cold, drought and salt stress with increases in cytoplasmic free calcium, there is currently no information about the role(s) of the functionally diverse cell types that comprise the root. Transgenic Arabidopsis with enhancer-trapped GAL4 expression inspecific cell types was used to target the calcium reporting protein, aequorin, fused to a modified yellow fluorescent protein (YFP). The luminescence output of targeted aequorin enabled in vivo measurement of changes in cytosolic free calcium concentrations ([Ca2+](cyt)) in specific cell types during acute cold, osmotic and salt stresses. In response to an acute cold stress, all cell types tested as well as plants constitutively expressing aequorin displayed rapid [Ca2+](cyt) peaks. However, there were significant quantitative differences between different cell types in terms of their response to cold stress, osmotic stress (440 mM mannitol) and salt stress (220 mMNaCl), implying specific roles for certain cell types in the detection and/or response to these stimuli. In response to osmotic and salt stress, the endodermis and pericycle displayed prolonged oscillations in cytosolic calcium that were distinct from the responses of the other cell types tested. Targeted expression of aequorin circumvented the technical difficulties involved in fluorescent dye injection as well as the lack of cell specificity of constitutively expressed aequorin, and revealed a new level of complexityin root calcium signalling.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 08:02:52