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Titolo:
Electrophysiological characteristics of non-bursting, glutamate decarboxylase messenger RNA-positive neurons of the medial septum/diagonal band nuclei of guinea-pig and rat
Autore:
Knapp, JA; Morris, NP; Henderson, Z; Matthews, RT;
Indirizzi:
Meharry Med Coll, Dept Anat & Physiol, Nashville, TN 37208 USA Meharry MedColl Nashville TN USA 37208 Physiol, Nashville, TN 37208 USA Univ Leeds, Sch Biomed Sci, Leeds LS2 9NQ, W Yorkshire, England Univ Leeds Leeds W Yorkshire England LS2 9NQ S2 9NQ, W Yorkshire, England
Titolo Testata:
NEUROSCIENCE
fascicolo: 4, volume: 98, anno: 2000,
pagine: 661 - 668
SICI:
0306-4522(2000)98:4<661:ECONGD>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACETYLTRANSFERASE-IMMUNOREACTIVE NEURONS; GAMMA-AMINOBUTYRIC-ACID; CHOLINE-ACETYLTRANSFERASE; BASAL FOREBRAIN; DIAGONAL BAND; SEPTOHIPPOCAMPAL NEURONS; INTRACELLULAR-RECORDINGS; INHIBITORY INTERNEURONS; CELL-TYPES; IN-VITRO;
Keywords:
medial septum; diagonal band; GABAergic neurons; f-AHP neurons; cholinergic neurons; glutamate decarboxylase mRNA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Matthews, RT Meharry Med Coll, Dept Anat & Physiol, 1005 DB Todd Blvd, Nashville, TN 37208 USA Meharry Med Coll 1005 DB Todd Blvd Nashville TN USA 37208 USA
Citazione:
J.A. Knapp et al., "Electrophysiological characteristics of non-bursting, glutamate decarboxylase messenger RNA-positive neurons of the medial septum/diagonal band nuclei of guinea-pig and rat", NEUROSCIENC, 98(4), 2000, pp. 661-668

Abstract

Nuclei of the medial septum/diagonal band region of the mammalian forebrain contain neurons that give rise to the septohippocampal pathway, which hasseparate cholinergic and GABAergic components. This pathway is known to influence hippocampal-dependent memory and learning processes, but the precise role of each component is unclear. In this study, we tested the hypothesis that fast-firing, non-bursting medial septum/diagonal band neurons are GABAergic. We used brain slice preparations from young adult guinea-pigs and rats, or from weanling rats, to perform current-clamp recordings from medial septum/diagonal band neurons. Recorded neurons were injected with biocytin for subsequent visualization with fluorescent avidin, and then hybridizedwith a S-35-labeled riboprobe for glutamate decarboxylase-67 messenger RNA. As a positive control, guinea-pig cerebellar Purkinje cells were labeled and hybridized with the riboprobe. As expected, labeled Purkinje cells wereglutamate decarboxylase-67 messenger RNA positive. Slow-firing, cholinergic (choline acetyltransferase-positive) guinea-pig medial septum/diagonal band neurons were glutamate decarboxylase-67 messenger RNA negative. Contraryto our hypothesis, of the guinea-pig neurons, only three of 11 fast-firingneurons were glutamate decarboxylase-67 positive. Of the rat medial septum/diagonal band neurons, three of four were positive for glutamate decarboxylase-67 messenger RNA. These data suggest that fast-firing, non-bursting neurons of the medial septum/diagonal band, as sampled by sharp-electrode intracellular recordings in brain slices, may be a heterogeneous group of neurons, some of which areGABAergic. Together with recent data demonstrating the presence of anotherGABAergic marker, parvalbumin, in fast-firing septal neurons, we conclude that GABAergic septohippocampal neurons include a population of fast-firing, non-bursting neurons. The influence of these neurons on the hippocampus is likely to occur on a shorter time-scale and over a wider range of firing frequencies as compared to slowly firing cholinergic septohippocampal neurons. (C) 2000 IBRO. Published by Elsevier Science Ltd. All rights reserved.

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Documento generato il 04/04/20 alle ore 00:38:07