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Titolo:
Alterations in bcl-2 and caspase gene family protein expression in human temporal lobe epilepsy
Autore:
Henshall, DC; Clark, RSB; Adelson, PD; Chen, M; Watkins, SC; Simon, RP;
Indirizzi:
Univ Pittsburgh, Dept Neurol, Pittsburgh, PA 15260 USA Univ Pittsburgh Pittsburgh PA USA 15260 Neurol, Pittsburgh, PA 15260 USA Univ Pittsburgh, Dept Anesthesiol & Crit Care Med, Pittsburgh, PA 15260 USA Univ Pittsburgh Pittsburgh PA USA 15260 are Med, Pittsburgh, PA 15260 USA Univ Pittsburgh, Dept Neurol Surg, Pittsburgh, PA 15260 USA Univ Pittsburgh Pittsburgh PA USA 15260 ol Surg, Pittsburgh, PA 15260 USA Univ Pittsburgh, Dept Physiol & Cell Biol, Pittsburgh, PA 15260 USA Univ Pittsburgh Pittsburgh PA USA 15260 ll Biol, Pittsburgh, PA 15260 USA
Titolo Testata:
NEUROLOGY
fascicolo: 2, volume: 55, anno: 2000,
pagine: 250 - 257
SICI:
0028-3878(20000725)55:2<250:AIBACG>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
SUSTAINED ELECTRICAL-STIMULATION; NONCONVULSIVE SEIZURES DAMAGE; TRAUMATIC BRAIN INJURY; CELL-DEATH; RAT-BRAIN; HIPPOCAMPAL-NEURONS; PERFORANT PATH; MESSENGER-RNA; KAINIC ACID; PROTOONCOGENE BCL-2;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
50
Recensione:
Indirizzi per estratti:
Indirizzo: Simon, RP Legacy Clin Res & Technol Ctr, 1225 NE 2nd Ave, Portland, OR 97208 USA Legacy Clin Res & Technol Ctr 1225 NE 2nd Ave Portland OR USA 97208
Citazione:
D.C. Henshall et al., "Alterations in bcl-2 and caspase gene family protein expression in human temporal lobe epilepsy", NEUROLOGY, 55(2), 2000, pp. 250-257

Abstract

Objective: To address the role of cell death regulatory genes of the bcl-2and caspase families in the neuropathology of human epilepsy using tissue extracted from patients undergoing temporal lobectomy for intractable seizures. Methods: Using Western blotting and immunohistochemistry, the authors investigated the expression of bcl-2, bcl-x(L), bar, caspase-1, and caspase-3 in temporal cortex samples from patients who had undergone temporal lobectomy surgery for intractable epilepsy (n = 19). Nonepileptic postmortem tissue from a brain bank served as control (n = 6). Results: Western blot analysis demonstrated significant increases in levels of bcl-2 and bcl-x(L) protein in seizure brain compared to control. Cleavage of caspase-1 was evidenced by a reduction in levels of the 45 kDa proenzyme form and an increase in levels of the p10 fragment. Levels of the 32 kDa proenzyme form of caspase-3 were elevated in seizure patients, as were levels of the 12 kDa cleaved fragment. Bcl-2, bar, and caspase-3 immunoreactivity was increased predominantly in cells with the morphologic appearance of neurons, whereas bcl-x(L) immunoreactivity was increased in cells with the appearance of glia. DNAfragmentation was detected in some but not all sections from epileptic brain samples. Conclusions: Cell death regulatory genes of the bcl-2 and caspase families may play a role in ongoing neuropathologic processes in human epilepsy, and offer novel targets as an adjunct to anticonvulsant therapy.

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Documento generato il 05/04/20 alle ore 06:16:20