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Titolo:
Repressor-AFLR interaction modulates aflatoxin biosynthesis in Aspergillusparasiticus
Autore:
Chang, PK; Yu, JJ; Bhatnagar, D; Cleveland, TE;
Indirizzi:
ARS, So Reg Res Ctr, USDA, New Orleans, LA 70124 USA ARS New Orleans LA USA 70124 Reg Res Ctr, USDA, New Orleans, LA 70124 USA
Titolo Testata:
MYCOPATHOLOGIA
fascicolo: 2, volume: 147, anno: 1999,
pagine: 105 - 112
SICI:
0301-486X(1999)147:2<105:RIMABI>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
REGULATORY PROTEIN; GENE-CLUSTER; AMINO-ACIDS; PATHWAY; NIDULANS; BINDING; DNA; FLAVUS; TRANSCRIPTION; EXPRESSION;
Keywords:
aflatoxin; aflR; Aspergillus parasiticus; repressor; sclerotia;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Chang, PK ARS, So Reg Res Ctr, USDA, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA ARS 1100 Robert E Lee Blvd New Orleans LA USA 70124 LA 70124 USA
Citazione:
P.K. Chang et al., "Repressor-AFLR interaction modulates aflatoxin biosynthesis in Aspergillusparasiticus", MYCOPATHOLO, 147(2), 1999, pp. 105-112

Abstract

Regulation of aflatoxin (AF) biosynthesis likely involves a complex interplay of positive- and negative-acting factors that are affected by physiological cues responsive to internal and external stimuli. These factors, presumably, modulate the expression of the AF pathway-specific regulatory gene, aflR, whose product, AFLR, a zinc cluster transcription factor, then turns on or off the transcription of other AF genes. To determine if the AFLR carboxyl region (AFLRC) interacts with positive- or negative-acting proteins, we fused the Aspergillus parasiticus aflR carboxyl coding region (aflRC) tothe promoter of A. parasiticus nitrite reductase gene (niiA(p)::aflRC), and transformed it into A. parasiticus SRRC 2043. Transformants that contained two copies of niiA(p)::aflRC, one at the niaD locus and another at the aflR locus, overproduced AF precursors independent of the nitrogen source. The higher copy number of the integrated niiA(p)::aflRC correlated with increased production of AF precursors by the transformants as well as increased expression of both aflRC and native aflR in potato dextrose broth and A & Mmedium. Since aflRC does not encode a DNA-binding domain, the expressed AFLRC should not bind to the promoters of AF pathway genes and affect transcription directly. The results are consistent with AFLRC titrating out a putative repressor that interacts with AFLR under different growth conditions and modulates AF biosynthesis. This interaction also indirectly affects sclerotial development.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/09/18 alle ore 08:05:10