Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Role of aquaporins in alveolar fluid clearance in neonatal and adult lung,and in oedema formation following acute lung injury: studies in transgenicaquaporin null mice
Autore:
Song, YL; Fukuda, N; Bai, CX; Ma, TH; Matthay, MA; Verkman, AS;
Indirizzi:
Univ Calif San Francisco, Cardiovasc Res Inst, Dept Med, San Francisco, CA94143 USA Univ Calif San Francisco San Francisco CA USA 94143 rancisco, CA94143 USA Univ Calif San Francisco, Cardiovasc Res Inst, Dept Physiol, San Francisco, CA 94143 USA Univ Calif San Francisco San Francisco CA USA 94143 ancisco, CA 94143 USA Shanghai Med Univ, Shanghai 200032, Peoples R China Shanghai Med Univ Shanghai Peoples R China 200032 00032, Peoples R China
Titolo Testata:
JOURNAL OF PHYSIOLOGY-LONDON
fascicolo: 3, volume: 525, anno: 2000,
pagine: 771 - 779
SICI:
0022-3751(20000615)525:3<771:ROAIAF>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
SURFACE FLUORESCENCE METHOD; INSENSITIVE WATER CHANNEL; INDUCED PULMONARY-EDEMA; RAT LUNG; PROTEIN CLEARANCE; PERINATAL-PERIOD; EPITHELIAL-CELLS; AIR SPACES; PERMEABILITY; TRANSPORT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
58
Recensione:
Indirizzi per estratti:
Indirizzo: Verkman, AS Univ Calif San Francisco, Cardiovasc Res Inst, Dept Med, 1246 Hlth Sci E Tower,Box 0521, San Francisco, CA 94143 USA Univ Calif San Francisco 1246 Hlth Sci E Tower,Box 0521 San Francisco CA USA 94143
Citazione:
Y.L. Song et al., "Role of aquaporins in alveolar fluid clearance in neonatal and adult lung,and in oedema formation following acute lung injury: studies in transgenicaquaporin null mice", J PHYSL LON, 525(3), 2000, pp. 771-779

Abstract

1. Aquaporin (AQP) water channels provide a major pathway for osmotically driven water movement across epithelial and microvascular barriers in the lung. We used mice deficient in each of the three principal lung aquaporins,AQP1, AQP4 and AQP5, to test the hypothesis that aquaporins are important in neonatal lung fluid balance, adult lung fluid clearance and formation oflung oedema after acute lung injury.2. Wet-to-dry weight ratios (W/D) in lungs from wild-type mice decreased from 7.9 to 5.7 over the first hour after spontaneous delivery. AQP deletiondid not significantly affect W/D at 45 min after birth.3. Alveolar fluid clearance was measured in living ventilated mice in which 0.5 ml saline containing radiolabelled albumin was instilled into the airspaces. Fluid clearance was 17.4% in 15 min and inhibited > 90 % by amiloride, but clearance was not affected by AQP deletion.4. W/D was measured in established models of acute lung injury - acid aspiration and thiourea administration. Two hours after intratracheal administration of HCl, W/D increased from 3.7 to 7.5 but was not affected by AQP deletion. Three hours after intraperitoneal infusion of thiourea, W/D increased to 5.5 and marked pleural effusions appeared, but there were no differences in wild-type and AQP knockout mice.5. Hyperoxic subacute lung injury was induced by 95% oxygen. Neither mean survival (143 h) nor W/D at 65 h (5.1) were significantly affected by AQP deletion.6. Despite their role in osmotically driven lung water transport, aquaporins are not required for the physiological clearance of lung water in the neonatal or adult lung, or for the accumulation of extravascular lung water in the injured lung.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/07/20 alle ore 21:59:11