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Titolo:
Functional involvement of E-cadherin in TGF-beta 1-induced cell cluster formation of in vitro developing human langerhans-type dendritic cells
Autore:
Riedl, E; Stockl, J; Majdic, O; Scheinecker, C; Rappersberger, K; Knapp, W; Strobl, H;
Indirizzi:
Univ Vienna, Inst Immunol, A-1090 Vienna, Austria Univ Vienna Vienna Austria A-1090 , Inst Immunol, A-1090 Vienna, Austria Novartis Res Inst, Vienna Int Res Cooperat Ctr, Inst Immunol, Vienna, Austria Novartis Res Inst Vienna Austria rat Ctr, Inst Immunol, Vienna, Austria Univ Vienna, Dept Internal Med 3, Div Rheumatol, A-1090 Vienna, Austria Univ Vienna Vienna Austria A-1090 Div Rheumatol, A-1090 Vienna, Austria Univ Vienna, Dept Dermatol 1, A-1090 Vienna, Austria Univ Vienna Vienna Austria A-1090 ept Dermatol 1, A-1090 Vienna, Austria
Titolo Testata:
JOURNAL OF IMMUNOLOGY
fascicolo: 3, volume: 165, anno: 2000,
pagine: 1381 - 1386
SICI:
0022-1767(20000801)165:3<1381:FIOEIT>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
CD34(+) HEMATOPOIETIC PROGENITORS; COLONY-STIMULATING FACTOR; MOLECULE E-CADHERIN; T-CELLS; TRANSFORMING GROWTH-FACTOR-BETA-1; ADHESION MOLECULE-1; EPITHELIAL-CELLS; FLT3 LIGAND; NULL MICE; TGF-BETA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
46
Recensione:
Indirizzi per estratti:
Indirizzo: Strobl, H Univ Vienna, Inst Immunol, Borschkegasse 8A, A-1090 Vienna, Austria Univ Vienna Borschkegasse 8A Vienna Austria A-1090 nna, Austria
Citazione:
E. Riedl et al., "Functional involvement of E-cadherin in TGF-beta 1-induced cell cluster formation of in vitro developing human langerhans-type dendritic cells", J IMMUNOL, 165(3), 2000, pp. 1381-1386

Abstract

Epithelial Langerhans cells (LC) represent immature dendritic cells that require TGF-beta 1 stimulation for their development. Little is known about the mechanisms regulating LC generation from their precursor cells. We demonstrate here that LC development from human CD34(+) hemopoietic progenitor cells in response to TGF-beta 1 costimulation (basic cytokine combination GM-CSF plus TNF-alpha, stem cell factor, and Flt3 ligand) is associated withpronounced cell cluster formation of developing LC precursor cells. This cell-clustering phenomenon requires hemopoietic progenitor cell differentiation, since it is first seen on day 4 after culture initiation of CD34(+) cells, Cell cluster formation morphologically indicates progenitor cell development along the LC pathway, because parallel cultures set up in the absence of exogenous TGF-beta 1 fail to form cell clusters and predominantly giverise to monocyte, but not LC, development (CD1a(-), lysozyme(+), CD14(+)). TGF-beta 1 costimulation of CD34(+) cells induces neoexpression of the homophilic adhesion molecule E-cadherin in the absence of the E-cadherin heteroligand CD103, Addition of anti-E-cadherin mAb or mAbs to any of the constitutively expressed adhesion molecule (CD99, CD31, LFA-1, or CD18) to TGP-beta 1-supplemented progenitor cell cultures inhibits LC precursor cell cluster formation, and this effect is, with the exception of anti-E-cadherin mAb, associated with inhibition of LC generation. Addition of anti-E-cadherin mAb to the culture allows cell cluster-independent generation of LC from CD34(+) cells. Thus, functional E-cadherin expression and homotypic cell cluster formation represent a regular response of LC precursor cells to TGF-beta 1 stimulation, and cytoadhesive interactions may modulate LC differentiation from hemopoietic progenitor cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/07/20 alle ore 00:02:05