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Titolo:
Transcriptional regulation of rat scavenger receptor class B type I gene
Autore:
Mizutani, T; Yamada, K; Minegishi, T; Miyamoto, K;
Indirizzi:
Fukui Med Univ, Dept Biochem, Fukui 9101193, Japan Fukui Med Univ Fukui Japan 9101193 v, Dept Biochem, Fukui 9101193, Japan Gunma Univ, Sch Med, Dept Obstet & Gynecol, Maebashi, Gumma 371, Japan Gunma Univ Maebashi Gumma Japan 371 & Gynecol, Maebashi, Gumma 371, Japan
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 29, volume: 275, anno: 2000,
pagine: 22512 - 22519
SICI:
0021-9258(20000721)275:29<22512:TRORSR>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
HIGH-DENSITY-LIPOPROTEIN; STEROIDOGENIC FACTOR-I; BOVINE CYP11A GENE; PROTEIN STAR GENE; APOPROTEIN-A-I; SR-BI; PROMOTER ACTIVITY; BINDING PROTEIN; ADRENAL-GLAND; EXPRESSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Miyamoto, K Fukui Med Univ, Dept Biochem, Fukui 9101193, Japan Fukui Med Univ Fukui Japan 9101193 hem, Fukui 9101193, Japan
Citazione:
T. Mizutani et al., "Transcriptional regulation of rat scavenger receptor class B type I gene", J BIOL CHEM, 275(29), 2000, pp. 22512-22519

Abstract

The scavenger receptor class B type I (SR-BI) mediates the selective transport of lipids from high density lipoprotein to cells and plays an important role in the reverse uptake of cholesterol to the liver and in the delivery of substrates for steroidogenesis in steroidogenic organs. We report hereon the isolation and characterization of the upstream promoter region of the rat SR-BI gene. The transcription start site for rat SR-BI was mapped, and DNA sequence analysis revealed the presence of binding sites for the Sp1family in the proximal 5'-flanking region. Analysis of deletion mutants with different 5' lengths revealed that the region between -121 and -90 base pairs from the transcription start site is essential for the efficient transcription of SR-BI. Both Sp1 and Sp3 bind to three GC boxes in the region (-141 to -1 base pairs) in a sequence-specific manner, Mutations in any of the GC boxes decreased efficient transcription from this promoter in MA-10 mouse Leydig tumor cells, The overexpression of Sp1 or Sp3 protein enhanced the rat SR-BI promoter activity. These results indicate that Sp1 family members of transcription factors are essential for transcription of the rat SR-BI gene.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 07:00:33