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Titolo:
The contribution of mitochondrial respiratory complexes to the production of reactive oxygen species
Autore:
McLennan, HR; Degli Esposti, M;
Indirizzi:
Monash Univ, Dept Biochem & Mol Biol, Clayton, Vic 3168, Australia Monash Univ Clayton Vic Australia 3168 Biol, Clayton, Vic 3168, Australia Univ Manchester, Sch Biol Sci, CRC Mol Pharmacol, Manchester M13 9PT, Lancs, England Univ Manchester Manchester Lancs England M13 9PT M13 9PT, Lancs, England
Titolo Testata:
JOURNAL OF BIOENERGETICS AND BIOMEMBRANES
fascicolo: 2, volume: 32, anno: 2000,
pagine: 153 - 162
SICI:
0145-479X(200004)32:2<153:TCOMRC>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
CYTOCHROME-C REDUCTASE; HYDROGEN-PEROXIDE GENERATION; CERAMIDE-INDUCED APOPTOSIS; NADH-UBIQUINONE REDUCTASE; BOVINE HEART-MITOCHONDRIA; ELECTRON-TRANSPORT CHAIN; TUMOR-NECROSIS-FACTOR; SUBMITOCHONDRIAL PARTICLES; SUPEROXIDE ANION; RADICAL GENERATION;
Keywords:
reactive oxygen species; mitochondria; respiratory inhibitors; ubiquinone;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
56
Recensione:
Indirizzi per estratti:
Indirizzo: McLennan, HR Monash Univ, Dept Biochem & Mol Biol, Clayton, Vic 3168, Australia Monash Univ Clayton Vic Australia 3168 , Vic 3168, Australia
Citazione:
H.R. McLennan e M. Degli Esposti, "The contribution of mitochondrial respiratory complexes to the production of reactive oxygen species", J BIOENER B, 32(2), 2000, pp. 153-162

Abstract

This work was focused on distinguishing the contribution of mitochondrial redox complexes to the production of reactive oxygen species (ROS) during cellular respiration. We were able to accurately measure, for the first time, the basal production of ROS under uncoupled conditions by using a very sensitive method, based on the fluorescent probe dichlorodihydrofluorescein diacetate. The method also enabled the detection of the ROS generated by theoxidation of the endogenous substrates in the mitochondrial preparations and could be applied to both mitochondria and live cells. Contrary to the commonly accepted view that complex III (ubiquinol:cytochrome c reductase) isthe major contributor to mitochondrial ROS production, we found that complex I (NADH-ubiquinone reductase) and complex It (succinate-ubiquinone reductase) are the predominant generators of ROS during prolonged respiration under uncoupled conditions. Complex II, in particular, appears to contribute to the basal production of ROS in cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/01/20 alle ore 12:41:47