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Titolo:
Characterization of vitellin and vitellogenin of giant tiger prawn Penaeusmonodon using monoclonal antibodies specific to vitellin subunits
Autore:
Longyant, S; Sithigorngul, P; Thammapalerd, N; Sithigorngul, W; Menasveta, P;
Indirizzi:
Srinakharinwirot Univ, Fac Sci, Dept Biol, Bangkok 10110, Thailand Srinakharinwirot Univ Bangkok Thailand 10110 ol, Bangkok 10110, Thailand Chulalongkorn Univ, Fac Sci, Dept Marine Sci, Bangkok 10330, Thailand Chulalongkorn Univ Bangkok Thailand 10330 e Sci, Bangkok 10330, Thailand Mahidol Univ, Fac Trop Med, Dept Microbiol & Immunol, Bangkok 10400, Thailand Mahidol Univ Bangkok Thailand 10400 l & Immunol, Bangkok 10400, Thailand Chulalongkorn Univ, Aquat Resources Res Inst, Bangkok 10330, Thailand Chulalongkorn Univ Bangkok Thailand 10330 Inst, Bangkok 10330, Thailand BIOTEC, Marine Biotechnol Res Unit, Bangkok 10400, Thailand BIOTEC Bangkok Thailand 10400 otechnol Res Unit, Bangkok 10400, Thailand
Titolo Testata:
INVERTEBRATE REPRODUCTION & DEVELOPMENT
fascicolo: 3, volume: 37, anno: 2000,
pagine: 211 - 221
SICI:
0792-4259(200005)37:3<211:COVAVO>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
MATURE FEMALE HEMOLYMPH; FRESH-WATER PRAWN; MACROBRACHIUM-ROSENBERGII; PROTEIN VITELLOGENIN; SEMISULCATUS DEHAAN; CELL-FUSION; PURIFICATION; OVARIES; SHRIMP; CRUSTACEA;
Keywords:
crustacean; monoclonal antibodies; Penaeus monodon; vitellin; vitellogenin; subunits; characterization;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Sithigorngul, P Srinakharinwirot Univ, Fac Sci, Dept Biol, Bangkok 10110, Thailand Srinakharinwirot Univ Bangkok Thailand 10110 0, Thailand
Citazione:
S. Longyant et al., "Characterization of vitellin and vitellogenin of giant tiger prawn Penaeusmonodon using monoclonal antibodies specific to vitellin subunits", INVERTEBR R, 37(3), 2000, pp. 211-221

Abstract

Monoclonal antibodies specific to Penaeus monodon vitellin subunits were produced from mouse immunized with sodium dodecyl sulfate (SDS) treated ovarian extract prepared fi om gravid ovaries. After fusion of mouse spleen cells with P3X myeloma, hybridomas were selected by indirect immunoperoxidase ELISA against P. monodon ovarian extract. This was followed by dot-blottingagainst native and denatured proteins from ovarian extract, female haemolymph, and male haemolymph, then by dot-blotting against each vitellin subunit. Hybridoma clones producing antibodies specific to each of vitellin subunits with a molecular mass of 83, 74, 104 and 58, 104 and 45 kD, antibodies specific to the 215 kD protein, an oocyte-specific protein, and one monoclonal antibody specific to haemocyanin were isolated. All monoclonal antibodies could bind to both native and denatured proteins. Western blot analysis of ovarian extract and female haemolymph from gravid ovary prawns separatedby PAGE and SDS-PAGE revealed five vitellin subunits, molecular mass of 104, 83, 74, 58 and 45 kD in ovarian extract, and four vitellogenin related polypeptides, molecular mass of 200, 104, 83 and 74 kD in the female haemolymph. From the immunoreactive relationships among these proteins, it could be assumed that vitellogenin may be released into the haemolymph in two forms, 200 and 74 kD, then the 200 kD polypeptide was either processed into the104 and 83 kD polypeptides, or directly taken up into the oocyte. In the oocyte, the 104 kD protein would be further cleaved into 58 and 45kD polypeptides while the 74 kD protein would undergo slight modification or remainedunchanged. Western blot analysis of vitellin subunits at various stages ofovarian development revealed that the 200 kD protein appeared in the oocyte during early ovarian development the 45 and 58 kD proteins appeared during the late development.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/09/20 alle ore 05:00:00