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Titolo:
Bax is downregulated in inflamed colonic mucosa of ulcerative colitis
Autore:
Iimura, M; Nakamura, T; Shinozaki, S; Iizuka, B; Inoue, Y; Suzuki, S; Hayashi, N;
Indirizzi:
Tokyo Womens Med Univ, Inst Gastroenterol, Shinjuku Ku, Tokyo 1628666, Japan Tokyo Womens Med Univ Tokyo Japan 1628666 njuku Ku, Tokyo 1628666, Japan
Titolo Testata:
GUT
fascicolo: 2, volume: 47, anno: 2000,
pagine: 228 - 235
SICI:
0017-5749(200008)47:2<228:BIDIIC>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROGRAMMED CELL-DEATH; INTESTINAL EPITHELIAL-CELLS; NITRIC-OXIDE; IN-VIVO; GASTROINTESTINAL-TRACT; CROHNS-DISEASE; BCL-2; APOPTOSIS; EXPRESSION; REGULATOR;
Keywords:
ulcerative colitis; apoptosis; Bax; Bcl-2; Bcl-xL; CD95;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
44
Recensione:
Indirizzi per estratti:
Indirizzo: Nakamura, T Tokyo Womens Med Univ, Inst Gastroenterol, Shinjuku Ku, 8-1 Kawada Cho, Tokyo 1628666, Japan Tokyo Womens Med Univ 8-1 Kawada Cho Tokyo Japan 1628666 apan
Citazione:
M. Iimura et al., "Bax is downregulated in inflamed colonic mucosa of ulcerative colitis", GUT, 47(2), 2000, pp. 228-235

Abstract

Background and aims-One form of epithelial cell injury in inflamed colonicmucosa in ulcerative colitis (UC) is reported to involve apoptosis of these cells. Bcl-2 family proteins Bar and Bcl-2 are the major regulators of apoptosis. The aim of this study was to elucidate the involvement of the Bax/Bcl-2 system in induction of apoptosis of the inflamed colonic epithelium in UC. Methods-Colonic epithelium was isolated from colonic biopsy specimens. Expression of CD95, Pax, Bcl-xL, and Bcl-2 proteins was determined by western blotting. Bar gene expression was assessed by both reverse transcription-polymerase chain reaction (RT-PCR) followed by Southern hybridisation and a real time PCR assay. Results-Equal levels of expression of CD95, Bcl-xL, and Bcl-2 proteins were noted in normal and UC colonic epithelia. Equal levels of expression of Pax protein and mRNA were noted in epithelia of normal colon and inactive UC. Levels of expression of Bax protein and mRNA were markedly reduced in inflamed UC colonic epithelium. Conclusions-Our study showed for the first time downregulation of Bar in inflamed colonic epithelium of UC. The Bax/Bcl-2 system did not seem to be involved in induction of apoptosis of epithelial cells in the inflamed colonic mucosa of UC.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/11/20 alle ore 10:47:38