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Titolo:
Use of an activation-specific probe to show that Rap1A and Rap1B display different sensitivities to activation by forskolin in Rat1 cells
Autore:
McPhee, I; Houslay, MD; Yarwood, SJ;
Indirizzi:
Univ Glasgow, IBLS, Div Biochem & Mol Biol, Mol Pharmacol Grp, Glasgow G128QQ, Lanark, Scotland Univ Glasgow Glasgow Lanark Scotland G12 8QQ gow G128QQ, Lanark, Scotland
Titolo Testata:
FEBS LETTERS
fascicolo: 3, volume: 477, anno: 2000,
pagine: 213 - 218
SICI:
0014-5793(20000721)477:3<213:UOAAPT>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
NUCLEOTIDE-EXCHANGE FACTOR; GTP-BINDING PROTEINS; CYCLIC-AMP; DEPENDENT ACTIVATION; RAS; INHIBITION; FAMILY; KINASE; PHOSPHORYLATION;
Keywords:
Rap1; cyclic AMP; cyclic AMP-dependent protein kinase; forskolin; CREB; small GTPase; guanine nucleotide exchange factor; exchange protein directly activated by cAMP; cAMP-dependent GTP exchange factor; cAMP-specific phosphodiesterase of the type 4 family; cAMP phosphodiesterase; Rolipram;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
21
Recensione:
Indirizzi per estratti:
Indirizzo: Yarwood, SJ Ontario Canc Inst, Div Expt Therapeut, Room 10-621,610 Univ Ave, Toronto, ON M5G 2M9, Canada Ontario Canc Inst Room 10-621,610 Univ Ave Toronto ON Canada M5G 2M9
Citazione:
I. McPhee et al., "Use of an activation-specific probe to show that Rap1A and Rap1B display different sensitivities to activation by forskolin in Rat1 cells", FEBS LETTER, 477(3), 2000, pp. 213-218

Abstract

Rap1A and Rap1B are small GTPases of the Pas superfamily whose activation can be measured using a probe that interacts specifically with the GTP-bound forms of Rap1A and Rap1B. Using this procedure we demonstrate that the cyclic AMP-elevating agent forskolin activates both Rap1A and Rap1B in Rat1 cells. Whilst the protein kinase A inhibitor H89 ablated the ability of forskolin to cause cAMP response element binding protein (CREB) phosphorylationin Rat1 cells, it did not affect the ability of forskolin to activate either Rap1A and Rap1B, Forskolin differentially activated Rap1A and Rap1B isoforms in a time- and dose-dependent manner. The cAMP-specific type 4 family phosphodiesterase inhibitor rolipram potentiated the rate of activation of both Rap1A and Rap1B by forskolin challenge of Rat1 cells. Challenge of Rat1 cells with rolipram alone was able to elicit the phosphorylation of CREB but not activation of either Rap1A or Rap1B. (C) 2000 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 18:43:10