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Titolo:
Paclitaxel exposure time determines the nature of the interaction with radiation in HeLa cells: the role of apoptosis
Autore:
Yildiz, F; Perez, R; Redpath, JL;
Indirizzi:
Univ Calif Irvine, Dept Radiat Oncol, Irvine, CA 92697 USA Univ Calif Irvine Irvine CA USA 92697 Radiat Oncol, Irvine, CA 92697 USA Hacettepe Univ, Dept Radiat Oncol, TR-06100 Ankara, Turkey Hacettepe UnivAnkara Turkey TR-06100 iat Oncol, TR-06100 Ankara, Turkey
Titolo Testata:
EUROPEAN JOURNAL OF CANCER
fascicolo: 11, volume: 36, anno: 2000,
pagine: 1426 - 1432
SICI:
0959-8049(200007)36:11<1426:PETDTN>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
IONIZING-RADIATION; IN-VITRO; TAXOL; CYTOTOXICITY; COMBINATION; SENSITIZER; SCHEDULE; THERAPY; CANCER; CYCLE;
Keywords:
paclitaxel; radiation; apoptosis; HeLa cells;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Redpath, JL Univ Calif Irvine, Dept Radiat Oncol, Irvine, CA 92697 USA Univ Calif Irvine Irvine CA USA 92697 l, Irvine, CA 92697 USA
Citazione:
F. Yildiz et al., "Paclitaxel exposure time determines the nature of the interaction with radiation in HeLa cells: the role of apoptosis", EUR J CANC, 36(11), 2000, pp. 1426-1432

Abstract

The purpose of the present study was to investigate further the mechanismsunderlying subadditive and superadditive interactions of radiation and paclitaxel treatments in vitro. The protocol studied was a fixed radiation treatment (7 Gy) followed either immediately or after a 10 h delay by paclitaxel treatment. Paclitaxel treatment was exposure to either a fixed dose of paclitaxel (10 nM) for varying time intervals up to 25 h, or varying doses of paclitaxel up to 20 nM for a fixed exposure time of 24 h. The cells used were HeLa. Cell survival was assessed by colony forming ability and apoptosis was measured by flow cytometry. The results show that when the paclitaxel exposure time was 24 h superadditive interactions were observed at all paclitaxel doses. A reasonable correlation between surviving fraction as measured by colony forming ability and apoptosis in the attached cells at the end of paclitaxel treatment was observed. The nature of the interaction of radiation and subsequent paclitaxel treatment is critically dependent on theduration of the paclitaxel treatment. (C) 2000 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/09/20 alle ore 07:54:27