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Titolo:
Identification of the lipophilic factor produced by macrophages that stimulates steroidogenesis
Autore:
Nes, WD; Lukyanenko, YO; Jia, ZH; Quideau, S; Howald, WN; Pratum, TK; West, RR; Hutson, JC;
Indirizzi:
Texas Tech Univ, Hlth Sci Ctr, Dept Cell Biol & Biochem, Lubbock, TX 79430USA Texas Tech Univ Lubbock TX USA 79430 Biol & Biochem, Lubbock, TX 79430USA Texas Tech Univ, Dept Chem & Biochem, Lubbock, TX 79430 USA Texas Tech Univ Lubbock TX USA 79430 hem & Biochem, Lubbock, TX 79430 USA Univ Bordeaux 1, Lab Chim Subst Vegetales, F-33405 Talence, France Univ Bordeaux 1 Talence France F-33405 egetales, F-33405 Talence, France Univ Washington, Dept Med Chem, Seattle, WA 98195 USA Univ Washington Seattle WA USA 98195 Dept Med Chem, Seattle, WA 98195 USA Univ Washington, Dept Chem, Seattle, WA 98195 USA Univ Washington SeattleWA USA 98195 on, Dept Chem, Seattle, WA 98195 USA Zymogenet Inc, Seattle, WA 98195 USA Zymogenet Inc Seattle WA USA 98195Zymogenet Inc, Seattle, WA 98195 USA
Titolo Testata:
ENDOCRINOLOGY
fascicolo: 3, volume: 141, anno: 2000,
pagine: 953 - 958
SICI:
0013-7227(200003)141:3<953:IOTLFP>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
OXYSTEROL-BINDING-PROTEIN; LEYDIG-CELLS; TESTICULAR MACROPHAGES; ENDOTHELIAL-CELLS; CDNA CLONING; FACTOR-I; CHOLESTEROL; 25-HYDROXYCHOLESTEROL; RAT; LYMPHOCYTE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Hutson, JC Texas Tech Univ, Hlth Sci Ctr, Dept Cell Biol & Biochem, Lubbock, TX 79430USA Texas Tech Univ Lubbock TX USA 79430 hem, Lubbock, TX 79430USA
Citazione:
W.D. Nes et al., "Identification of the lipophilic factor produced by macrophages that stimulates steroidogenesis", ENDOCRINOL, 141(3), 2000, pp. 953-958

Abstract

Macrophages are known to release a lipophilic factor that stimulates testosterone production by Leydig cells. This macrophage-derived factor (MDF) isthought to be physiologically relevant, because removal of macrophages from the testis results in altered testosterone secretion and reduced fertility. The purpose of the present study was to purify this factor, elucidate its chemical structure, and determine whether it is both present in the testis and acts when injected intratesticularly. Culture media from testicular and peritoneal macrophages were extracted with ether, and the organic phase was sequentially purified on C-18, silica, and cyano-HPLC columns. MDF was detected using a rat Leydig cell bioassay, with testosterone secretion being the end point. Purified material and crude ether extracts were analyzed by gas chromatography/mass spectrometry and nuclear magnetic resonance spectroscopy. The time of elution of MDF from both testicular and peritoneal macrophages was when MDF, obtained from the final HPLC column, was analyzed bygas chromatography. The MS fragmentation pattern of purified ma -terial from both peritoneal and testicular macrophages was identical to that of a reference preparation of 25-hydroxycholesterol. Also, the nuclear magnetic resonance spectrum of MDF was similar to that of authentic 25-hydroxycholesterol. When 25-hydroxycholesterol was subjected to the identical purificationscheme as MDF, it was found to elute at the same times as MDF on all threecolumns and elicited activity in the Leydig cell bioassay as expected. Control medium purified identically did not contain 25-hydroxycholesterol or have biological activity. Ether extracts of testis contained 25-hydroxycholesterol, indicating that this compound is present under physiological conditions; Similarly, when 25-hydroxycholesterol was injected into the testis ofadult rats, testosterone production was increased within 3 h. Taken together, these data indicate that the lipophilic factor produced by macrophages that stimulates steroidogenesis is 25-hydroxycholesterol.

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Documento generato il 07/07/20 alle ore 10:54:42