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Titolo:
Differential cloning of growth hormone-regulated hepatic transcripts in the aged rat
Autore:
Tollet-Egnell, P; Flores-Morales, A; Odeberg, J; Lundeberg, J; Norstedt, G;
Indirizzi:
Karolinska Hosp, Karolinska Inst, Dept Mol Med, S-17176 Stockholm, Sweden Karolinska Hosp Stockholm Sweden S-17176 Med, S-17176 Stockholm, Sweden Royal Inst Technol, Dept Biochem & Biotechnol, S-10044 Stockholm, Sweden Royal Inst Technol Stockholm Sweden S-10044 l, S-10044 Stockholm, Sweden
Titolo Testata:
ENDOCRINOLOGY
fascicolo: 3, volume: 141, anno: 2000,
pagine: 910 - 921
SICI:
0013-7227(200003)141:3<910:DCOGHH>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
GLUTATHIONE-S-TRANSFERASE; D-DOPACHROME TAUTOMERASE; MIGRATION INHIBITORY FACTOR; MITOCHONDRIAL ATP SYNTHASE; GH-REPLACEMENT THERAPY; ACETYL-COA CARBOXYLASE; COENZYME-A CARBOXYLASE; FATTY-ACID SYNTHESIS; GENE-EXPRESSION; FACTOR-I;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
73
Recensione:
Indirizzi per estratti:
Indirizzo: Tollet-Egnell, P Karolinska Hosp, Karolinska Inst, Dept Mol Med, CMM L801,S-17176 Stockholm, Sweden Karolinska Hosp CMM L801 Stockholm Sweden S-17176 Sweden
Citazione:
P. Tollet-Egnell et al., "Differential cloning of growth hormone-regulated hepatic transcripts in the aged rat", ENDOCRINOL, 141(3), 2000, pp. 910-921

Abstract

It has been suggested that aging or at least some of its symptoms are related to a physiological decline in GH levels with age. This study was performed to elucidate age-related changes in GH-dependent effects at the level of gene expression. Through the application of complementary DNA representational difference analysis (RDA) we have identified gene products that are reduced during aging in rat liver. The expression of these genes was restored upon GH treatment. Results from reverse Northern and ribonuclease protection analysis confirmed that the RDA products were truly differentially expressed. In addition to well characterized GH-regulated genes, including CYP2C12, CYP2C13, and alpha(2u)-globulin, we demonstrate the differential expression of at least 11 genes previously not known to be under GH control. Several hepatic transcripts encoding enzymes and receptors involved in the metabolism of protein, carbohydrates, and lipids were identified. Other RDA products consisted of transcripts encoding proteins involved in ATP synthesis, detoxification of reactive oxygen species, or immune responses. This listof GH-regulated genes in the old rat may shed further light on the action and mechanism behind the positive effects of GH on, for example, body composition and the immune system that have been observed in different animal and human studies.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/09/20 alle ore 22:20:44