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Titolo:
Long-term alterations in adiposity affect the expression of melanin-concentrating hormone and enkephalin but not proopiomelanocortin in the hypothalamus of ovariectomized ewes
Autore:
Henry, BA; Tilbrook, AJ; Dunshea, FR; Rao, A; Blache, D; Martin, GB; Clarke, IJ;
Indirizzi:
Monash Univ, Prince Henrys Inst Med Res, Clayton, Vic 3168, Australia Monash Univ Clayton Vic Australia 3168 Res, Clayton, Vic 3168, Australia Monash Univ, Dept Physiol, Clayton, Vic 3168, Australia Monash Univ Clayton Vic Australia 3168 siol, Clayton, Vic 3168, Australia Victorial Inst Anim Sci, Werribee, Vic 3030, Australia Victorial Inst AnimSci Werribee Vic Australia 3030 , Vic 3030, Australia Univ Western Australia, Fac Agr, Nedlands, WA 6009, Australia Univ WesternAustralia Nedlands WA Australia 6009 nds, WA 6009, Australia
Titolo Testata:
ENDOCRINOLOGY
fascicolo: 4, volume: 141, anno: 2000,
pagine: 1506 - 1514
SICI:
0013-7227(200004)141:4<1506:LAIAAT>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
MESSENGER-RNA LEVELS; NEUROPEPTIDE-Y; LUTEINIZING-HORMONE; GROWTH-HORMONE; BODY-WEIGHT; FOOD RESTRICTION; ANOREXIA-NERVOSA; FEEDING-BEHAVIOR; GENE-EXPRESSION; OPIOID-PEPTIDES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
56
Recensione:
Indirizzi per estratti:
Indirizzo: Clarke, IJ Monash Med Ctr, Prince Henry Inst, Level 4,Block E,346 Clayton Rd,POB 5152, Clayton, Vic 3168, Australia Monash Med Ctr Level 4,Block E,346 Clayton Rd,POB 5152 Clayton Vic Australia 3168
Citazione:
B.A. Henry et al., "Long-term alterations in adiposity affect the expression of melanin-concentrating hormone and enkephalin but not proopiomelanocortin in the hypothalamus of ovariectomized ewes", ENDOCRINOL, 141(4), 2000, pp. 1506-1514

Abstract

We have developed a ruminant model to study long-term alterations in adiposity on the expression of appetite-regulating peptides in the hypothalamus. In this model endocrine and metabolic status are fully defined as well as body composition. The current study sought to define the effects of alteredadiposity on the expression of genes for neuropeptide Y (NPY), POMC, enkephalin (ENK), and melanin-concentrating hormone (MCH). Ovariectomized ewes with high (60 +/- 1 kg) (FAT) or low (37 +/- 3 kg) body weights (THIN) were blood sampled every 10 min for 8 h to determine metabolic and endocrine status. The animals were then killed and the brains perfused for in situ hybridization. Body composition analysis was performed on the carcass using dualenergy x-ray absorptiometry; this indicated that the FAT animals were 36 +/- 1% fat, whereas the THIN animals were 15 +/- 2% fat. The LH interpulse interval was lower and mean GH concentrations were higher in the THIN animals; cortisol and TSH levels were not different between the two groups but free T-4 and free T-3 levels were lower; the FT3:FT4 ratio was higher in THINewes. Levels of insulin, lactate, and nonesterified fatty acids were lowerin the THIN group, and plasma glucose and urea concentrations were similarin THIN and FAT animals. Levels of gene expression of NPY and MCH were higher in THIN ewes. POMC expression was similar in the two groups. In the THIN animals, ENK expression was lower in the paraventricular and ventromedialnuclei but higher in the periventricular region. In conclusion, we have shown that alterations in adiposity influence the expression of appetite-regulating peptides in the absence of ovarian steroids. The appetite stimulators, NPY and MCH, appear to be involved in the metabolic response to altered adiposity, whereas ENK in the periventricular region may be linked to the secretion of GH and possibly LH. Our results suggest that altered expressionof appetite- regulating peptides can be linked with the endocrine and metabolic adaptations that occur with long-term changes in adiposity.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/09/20 alle ore 22:08:03