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Titolo:
Steroidogenic factor-1 influences protein-deoxyribonucleic acid interactions within the cyclic adenosine 3 ',5 '-monophosphate-responsive regions of the murine steroidogenic acute regulatory protein gene
Autore:
Wooton-Kee, CR; Clark, BJ;
Indirizzi:
Univ Louisville, Sch Med, Dept Biochem & Mol Biol, Louisville, KY 40292 USA Univ Louisville Louisville KY USA 40292 ol Biol, Louisville, KY 40292 USA
Titolo Testata:
ENDOCRINOLOGY
fascicolo: 4, volume: 141, anno: 2000,
pagine: 1345 - 1355
SICI:
0013-7227(200004)141:4<1345:SFIPAI>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
SIDE-CHAIN CLEAVAGE; TRANSCRIPTION FACTORS GATA-4; LIPOID ADRENAL-HYPERPLASIA; NUCLEAR RECEPTOR SF-1; LEYDIG TUMOR-CELLS; FACTOR-I; PROMOTER ACTIVITY; STAR GENE; GRANULOSA-CELLS; EXPRESSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Clark, BJ Univ Louisville, Sch Med, Dept Biochem & Mol Biol, Louisville, KY 40292 USA Univ Louisville Louisville KY USA 40292 ouisville, KY 40292 USA
Citazione:
C.R. Wooton-Kee e B.J. Clark, "Steroidogenic factor-1 influences protein-deoxyribonucleic acid interactions within the cyclic adenosine 3 ',5 '-monophosphate-responsive regions of the murine steroidogenic acute regulatory protein gene", ENDOCRINOL, 141(4), 2000, pp. 1345-1355

Abstract

De novo synthesis of the steroidogenic acute regulatory protein (StAR) in response to trophic hormonal stimulation of steroidogenic cells is requiredfor the delivery of cholesterol from the mitochondrial outer membrane to the mitochondrial inner membrane and the cytochrome P450 side-chain cleavageenzyme. StAR expression is transcriptionally regulated by cAMP-mediated mechanisms, and we have identified a 45-bp region within the mouse promoter that is important for cAMP responsiveness of the gene. This region, located between -105 and -60 of the start site of transcription, contains a SF-1-binding site, a highly conserved C/EBP beta - AP-1-nuclear receptor half-sitesequences (CAN region), and a GATA-4-binding site. The SF-1 element and CAN region are required for full basal activity, whereas the GATA-4 element may account for 20% of the cAMP response in MA-10 mouse Leydig tumor cells. A cAMP-dependent protein-DNA complex was observed with the CAN region and mutation of a nonconsensus AP-1 site within this region greatly diminished promoter strength. Complex protein-DNA interactions within the cAMP responseregion (-105/-60) were shown to require the SF-1 element (-95), suggestingthat SF-1 is required for protein-DNA interaction at the CAN (-79) region and maximal activity of the promoter.

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Documento generato il 27/09/20 alle ore 21:44:06