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Titolo:
Agouti-related protein-like immunoreactivity: Characterization of release from hypothalamic tissue and presence in serum
Autore:
Li, JY; Finniss, S; Yang, YK; Zeng, Q; Qu, SY; Barsh, G; Dickinson, C; Gantz, I;
Indirizzi:
Univ Michigan, Dept Surg, Ann Arbor, MI 48109 USA Univ Michigan Ann ArborMI USA 48109 , Dept Surg, Ann Arbor, MI 48109 USA Univ Michigan, Dept Pediat, Ann Arbor, MI 48109 USA Univ Michigan Ann Arbor MI USA 48109 Dept Pediat, Ann Arbor, MI 48109 USA Stanford Univ, Howard Hughes Med Inst, Stanford, CA 94305 USA Stanford Univ Stanford CA USA 94305 ghes Med Inst, Stanford, CA 94305 USA Stanford Univ, Dept Pediat, Stanford, CA 94305 USA Stanford Univ StanfordCA USA 94305 , Dept Pediat, Stanford, CA 94305 USA Stanford Univ, Dept Genet, Stanford, CA 94305 USA Stanford Univ Stanford CA USA 94305 v, Dept Genet, Stanford, CA 94305 USA
Titolo Testata:
ENDOCRINOLOGY
fascicolo: 6, volume: 141, anno: 2000,
pagine: 1942 - 1950
SICI:
0013-7227(200006)141:6<1942:APICOR>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
NEUROPEPTIDE-Y RELEASE; PARAVENTRICULAR NUCLEUS; MELANOCORTIN RECEPTORS; FRAMESHIFT MUTATION; IN-VIVO; RAT; LEPTIN; OBESITY; FOOD; INCREASES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Gantz, I 6504 MSRBI,1150 W Med Ctr Dr, Ann Arbor, MI 48109 USA 6504 MSRBI,1150 W Med Ctr Dr Ann Arbor MI USA 48109 MI 48109 USA
Citazione:
J.Y. Li et al., "Agouti-related protein-like immunoreactivity: Characterization of release from hypothalamic tissue and presence in serum", ENDOCRINOL, 141(6), 2000, pp. 1942-1950

Abstract

A novel RIA was used to examine the release of agouti-related protein-likeimmunoreactivity (AGRP-LI) from perfused rat hypothalamic tissue slices and to characterize AGRP-LI in rat serum. A continuous low level basal AGRP-LI release was observed from hypothalami of rats fed ad libitum before the rats were killed. Basal AGRP-LI release was 3-fold greater in rats fasted 48h. In fasted animals leptin dose-dependently suppressed basal AGRP-LI release. In fed animals no change in basal AGRP-LI release was detected in response to 10(-6) M alpha-MSH, orexin B, melanin-concentrating hormone, or serotonin. HPLC analysis of AGRP-LI in rat serum identified a single peak thateluted in close proximity to synthetic AGRP (87-132) and mouse [Leu127Pro]AGRP and that was identical to the peak seen in hypothalamic and adrenal tissue extracts. The serum concentration of AGRP-LI in rats fed ad libitum was 0.865 +/- 0.323 nmol/liter (mean +/- SE). Food deprivation resulted in a slow, but statistically significant rise in serum immunoreactivity at 48 h [1.174 +/- 0.118 nmol/liter (mean +/- SE)]. Bilateral adrenalectomy did notchange serum levels of AGRP-LI. These studies demonstrate that in the rat there are different levels of basal hypothalamic AGRP-LI release in fed andfasted states and that in the fasted rat this release can be profoundly suppressed by leptin. These studies also suggest that AGRP is present in the systemic circulation of rats.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 23:01:52