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Titolo:
The distribution of cells containing estrogen receptor-alpha (ER alpha) and ER beta messenger ribonucleic acid in the preoptic area and hypothalamus of the sheep: Comparison of males and females
Autore:
Scott, CJ; Tilbrook, AJ; Simmons, DM; Rawson, JA; Chu, S; Fuller, PJ; Ing, NH; Clarke, IJ;
Indirizzi:
Prince Henrys Inst Med Res, Clayton, Vic 3168, Australia Prince Henrys Inst Med Res Clayton Vic Australia 3168 Vic 3168, Australia Univ So Calif, Dept Biol Sci, Los Angeles, CA 90089 USA Univ So Calif LosAngeles CA USA 90089 iol Sci, Los Angeles, CA 90089 USA Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA Texas A&M Univ College Stn TX USA 77843 im Sci, College Stn, TX 77843 USA
Titolo Testata:
ENDOCRINOLOGY
fascicolo: 8, volume: 141, anno: 2000,
pagine: 2951 - 2962
SICI:
0013-7227(200008)141:8<2951:TDOCCE>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
LUTEINIZING-HORMONE SECRETION; GONADOTROPIN-RELEASING-HORMONE; RAT-BRAIN; IMMUNOREACTIVE CELLS; TYROSINE-HYDROXYLASE; OVARIECTOMIZED EWE; GENE-EXPRESSION; NEURONS; CLONING; ESTRADIOL;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Citazione:
C.J. Scott et al., "The distribution of cells containing estrogen receptor-alpha (ER alpha) and ER beta messenger ribonucleic acid in the preoptic area and hypothalamus of the sheep: Comparison of males and females", ENDOCRINOL, 141(8), 2000, pp. 2951-2962

Abstract

We have used in situ hybridization to compare the distributions of estrogen receptor alpha (ER alpha) and ER beta messenger RNA (mRNA)-containing cells in the preoptic area and hypothalamus of ewes and rams. Perfusion-fixed brain tissue was collected from luteal phase ewes and intact rams (n = 4) during the breeding season. Matched pairs of sections were hybridized with sheep-specific, S-35-labeled riboprobes, and semiquantitative image analysiswas performed on emulsion-dipped slides. A number of sex differences were observed, with females having a greater density of labeled cells than males(P < 0.001) and a greater number of silver grains per cell (P < 0.01) in the ventromedial nucleus for both ER subtypes. In addition, in the retrochiasmatic area, males had a greater (P < 0.05) cell density for ER alpha mRNA-containing cells than females, whereas in the paraventricular nucleus, females had a greater density (P < 0.05) of ER alpha mRNA-containing cells thanmales. There was a trend (P = 0.068) in the arcuate nucleus for males to have a greater number of silver grains per cell labeled for ER alpha mRNA. In both sexes, there was considerable overlap in the distributions of ER alpha and ER beta mRNA-containing cells, but the density of labeled cells within each nucleus differed in a number of instances. Nuclei that contained a higher (P < 0.001) density of ER alpha than ER beta mRNA-containing cells included the preoptic area, bed nucleus of the stria terminalis, and ventromedial nucleus, whereas the subfornical organ (P < 0.001), paraventricular nucleus (males only, P < 0.05), and retrochiasmatic nucleus (females only, P< 0.05) had a greater density of ER alpha than ER beta mRNA-containing cells. The anterior hypothalamic area and supraoptic nucleus had similar densities of cells containing both ER subtypes. The lateral septum and arcuate nucleus contained only ER alpha, whereas only ER beta mRNA-containing cells were seen in the zona incerta. The sex differences in the populations of ERmRNA-containing cells in the ventromedial and arcuate nuclei may explain in part the sex differences in the neuroendocrine and behavioral responses to localized estrogen treatment in these nuclei. Within sexes, the differences between the distributions of ER alpha and ER beta mRNA-containing cells may reflect differential regulation of the actions of estrogen in the sheephypothalamus. Low levels of ER beta mRNA in the preoptic area and ventromedial and arcuate nuclei, regions known to be important for the regulation of reproduction, suggest that ER beta may not be involved in these functions.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/09/20 alle ore 08:32:36