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Titolo:
Sp1 and SF-1 interact and cooperate in the regulation of human steroidogenic acute regulatory protein gene expression
Autore:
Sugawara, T; Saito, M; Fujimoto, S;
Indirizzi:
Hokkaido Univ, Sch Med, Dept Biochem, Kita Ku, Sapporo, Hokkaido 0608638, Japan Hokkaido Univ Sapporo Hokkaido Japan 0608638 oro, Hokkaido 0608638, Japan Hokkaido Univ, Sch Med, Dept Obstet & Gynecol, Kita Ku, Sapporo, Hokkaido 0608638, Japan Hokkaido Univ Sapporo Hokkaido Japan 0608638 oro, Hokkaido 0608638, Japan Natl Canc Ctr, Res Inst, Div Virol, Tokyo 1040045, Japan Natl Canc Ctr Tokyo Japan 1040045 Inst, Div Virol, Tokyo 1040045, Japan
Titolo Testata:
ENDOCRINOLOGY
fascicolo: 8, volume: 141, anno: 2000,
pagine: 2895 - 2903
SICI:
0013-7227(200008)141:8<2895:SASIAC>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
LIPOID ADRENAL-HYPERPLASIA; FACTOR-I SF-1; TRANSCRIPTION FACTOR; PROMOTER ACTIVITY; STAR GENE; C/EBP-BETA; CYCLIC-AMP; RECEPTOR; CELLS; ACTIVATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Sugawara, T Hokkaido Univ, Sch Med, Dept Biochem, Kita Ku, Kita 15,Nishi 7, Sapporo, Hokkaido 0608638, Japan Hokkaido Univ Kita 15,Nishi 7 Sapporo Hokkaido Japan 0608638 n
Citazione:
T. Sugawara et al., "Sp1 and SF-1 interact and cooperate in the regulation of human steroidogenic acute regulatory protein gene expression", ENDOCRINOL, 141(8), 2000, pp. 2895-2903

Abstract

Steroidogenic acute regulatory (StAR) protein plays a critical role in themovement of cholesterol from the outer to the inner mitochondrial membrane. Steroidogenic factor 1 (SF-1) controls basal and cAMP-stimulated transcription of the StAR gene. The 1.3-kb StAR promoter has three SF-l binding sites, and two consensus transcription factor Spl binding sequences near the two most distal SF-1 binding sites. Spl mediates cAMP-dependent transcription of steroidogenic P450 enzyme genes,raising the possibility of Spl involvement in cAMP regulation of the StAR gene. However, the mechanism of Spl-mediated, cAMP-stimulated responsiveness is not known. In this study, we elucidated the roles of Sp1 and SF-1 in the regulation of the human StAR gene promoter. We found that there was negligible promoter activity in a pGL(2) StAR construct (-235 to +39) in which Spl and SF-1 binding sites were mutatedin Y-1 adrenal tumor cells. An Spl binding site mutation (pGL(2)Sp1M) did not support promoter activity, suggesting that Spl cooperates with SF-l in regulating StAR promoter function. In gel shift assays, the SF-1 binding site formed a complex with an SF-1-GST fusion protein and Spl. Coimmunoprecipitation cross-linking experiments indicated that SF-1 physically interacts with Spl in vitro. Finally, a mammalian two-hybrid system was employed to demonstrate that Spl and SF-1 associate in vivo. In conclusion, our data indicate that Spl and SF-1 physically interact and cooperate in the regulationof human StAR promoter activity.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/09/20 alle ore 14:09:12