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Titolo:
Corticotropin-releasing hormone type I receptor messenger ribonucleic acidand protein levels in the ovine fetal pituitary: Ontogeny and effect of chronic cortisol administration
Autore:
Green, JL; Figueroa, JP; Massmann, GA; Schwartz, J; Rose, JC;
Indirizzi:
Wake Forest Univ, Sch Med, Dept Obstet & Gynecol, Winston Salem, NC 27157 USA Wake Forest Univ Winston Salem NC USA 27157 , Winston Salem, NC 27157 USA Wake Forest Univ, Sch Med, Dept Physiol & Pharmacol, Winston Salem, NC 27157 USA Wake Forest Univ Winston Salem NC USA 27157 , Winston Salem, NC 27157 USA Wake Forest Univ, Sch Med, Perinatal Res Lab, Winston Salem, NC 27157 USA Wake Forest Univ Winston Salem NC USA 27157 , Winston Salem, NC 27157 USA
Titolo Testata:
ENDOCRINOLOGY
fascicolo: 8, volume: 141, anno: 2000,
pagine: 2870 - 2876
SICI:
0013-7227(200008)141:8<2870:CHTIRM>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
CHEMICAL CROSS-LINKING; RAT ANTERIOR-PITUITARY; FACTOR-BINDING-PROTEIN; FACTOR CRF RECEPTORS; ARGININE-VASOPRESSIN; ADRENOCORTICOTROPIN RELEASE; DEVELOPMENTAL-CHANGES; INTERMEDIATE LOBE; MOUSE PITUITARY; SHEEP ANTERIOR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Rose, JC Wake Forest Univ, Sch Med, Dept Obstet & Gynecol, Winston Salem, NC 27157 USA Wake Forest Univ Winston Salem NC USA 27157 Salem, NC 27157 USA
Citazione:
J.L. Green et al., "Corticotropin-releasing hormone type I receptor messenger ribonucleic acidand protein levels in the ovine fetal pituitary: Ontogeny and effect of chronic cortisol administration", ENDOCRINOL, 141(8), 2000, pp. 2870-2876

Abstract

In sheep, the ACTH secretory response to CRH in vivo or in vitro changes as a function of development, with peak responses occurring several weeks before term (145 days of gestation). CRH-stimulated ACTH secretion is mediated via the G protein-coupled CRH type I (CRH R1) receptor. We used a quantitative ribonuclease protection assay and Western immunoblotting to determinemessenger RNA (mRNA) and protein levels of the CRH R1 receptor in immatureand mature fetuses and adults. In addition, we precociously elevated fetalplasma cortisol levels to determine whether the fetal CRH R1 receptor is sensitive to increases in plasma cortisol. CRH R1 receptor mRNA levels decreased markedly throughout gestation and into the transition to adult life (immature fetus, 1.24 +/- 0.17; mature fetus, 0.75 +/- 0.13; adult, 0.18 +/- 0.093 pg/mu g total anterior pituitary RNA). Also, continuous cortisol infusion in immature fetuses significantly decreased CRH R1 mRNA levels by 41%. Similar decreases were noted in protein levels. Thus, the decreased ACTH response to CRH stimulation during late gestation may be related to decreased CRH R1 receptor expression. In addition, plasma cortisol levels may influence corticotroph responsiveness to CRH by decreasing CRH R1 receptor expression.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 22:22:11