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Titolo:
Distinct roles of two Yth1p domains in 3 '-end cleavage and polyadenylation of yeast pre-mRNAs
Autore:
Barabino, SML; Ohnacker, M; Keller, M;
Indirizzi:
Univ Basel, Bioctr, Dept Cell Biol, CH-4056 Basel, Switzerland Univ BaselBasel Switzerland CH-4056 ll Biol, CH-4056 Basel, Switzerland
Titolo Testata:
EMBO JOURNAL
fascicolo: 14, volume: 19, anno: 2000,
pagine: 3778 - 3787
SICI:
0261-4189(20000717)19:14<3778:DROTYD>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
MESSENGER-RNA 3'-ENDS; TAIL LENGTH CONTROL; SACCHAROMYCES-CEREVISIAE; SPECIFICITY FACTOR; POLY(A) POLYMERASE; MAMMALIAN CLEAVAGE; FACTOR-I; PROCESSING FACTOR; BINDING PROTEIN; COMPONENT;
Keywords:
mRNA; pre-mRNA 3 '-end processing; RNA-protein interaction; zinc finger;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Barabino, SML Univ Basel, Bioctr, Dept Cell Biol, Klingelbergstr 70, CH-4056 Basel, Switzerland Univ Basel Klingelbergstr 70 Basel Switzerland CH-4056 land
Citazione:
S.M.L. Barabino et al., "Distinct roles of two Yth1p domains in 3 '-end cleavage and polyadenylation of yeast pre-mRNAs", EMBO J, 19(14), 2000, pp. 3778-3787

Abstract

Yth1p is the yeast homologue of the 30 kDa subunit of mammalian cleavage and polyadenylation specificity factor (CPSF), The protein is part of the cleavage and polyadenylation factor CPF, which includes cleavage factor II (CF II) and polyadenylation factor I (PF I), and is required for both steps in pre-mRNA 3'-end processing, Yth1p is an RNA-binding protein that was previously shown to be essential for polyadenylation, Here, we demonstrate thatYth1p is also required for the cleavage reaction and that two protein domains have distinct roles in 3'-end processing. The C-terminal part is required in polyadenylation to tether Fip1p and poly(A) polymerase to the rest ofCPF, A single point mutation in the highly conserved second zinc finger impairs both cleavage and polyadenylation, and affects the ability of Yth1p to interact with the pre-mRNA and other CPF subunits, Finally, we find that Yth1p binds to CYC1 pre-mRNA in the vicinity of the cleavage site. Our results indicate that Yth1p is important for the integrity of CPF and participates in the recognition of the cleavage site.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 06:37:57