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Titolo:
Down-regulation of the inositol 1,4,5-trisphosphate receptor in mouse eggsfollowing fertilization or parthenogenetic activation
Autore:
Jellerette, T; He, CL; Wu, H; Parys, JB; Fissore, RA;
Indirizzi:
Univ Massachusetts, Dept Vet & Anim Sci, Paige Labs, Amherst, MA 01003 USAUniv Massachusetts Amherst MA USA 01003 Paige Labs, Amherst, MA 01003 USA Katholieke Univ Leuven, Fysiol Lab, Louvain, Belgium Katholieke Univ Leuven Louvain Belgium en, Fysiol Lab, Louvain, Belgium
Titolo Testata:
DEVELOPMENTAL BIOLOGY
fascicolo: 2, volume: 223, anno: 2000,
pagine: 238 - 250
SICI:
0012-1606(20000715)223:2<238:DOTI1R>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
INDUCED CALCIUM-RELEASE; GOLDEN-HAMSTER EGGS; CA2+ RELEASE; PHOSPHOLIPASE-C; BOVINE OOCYTES; TRISPHOSPHATE RECEPTORS; INTRACELLULAR CALCIUM; DEVELOPMENTAL-CHANGES; MUSCARINIC RECEPTOR; MEIOTIC MATURATION;
Keywords:
[Ca2+]i oscillations; oocytes; mammalian eggs; sperm factor; adenophostin A; thimerosal; strontium chloride; proteasome;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
71
Recensione:
Indirizzi per estratti:
Indirizzo: Fissore, RA Univ Massachusetts, Dept Vet & Anim Sci, Paige Labs, Amherst, MA 01003 USA Univ Massachusetts Amherst MA USA 01003 Amherst, MA 01003 USA
Citazione:
T. Jellerette et al., "Down-regulation of the inositol 1,4,5-trisphosphate receptor in mouse eggsfollowing fertilization or parthenogenetic activation", DEVELOP BIO, 223(2), 2000, pp. 238-250

Abstract

Fertilization in mammalian eggs is characterized by the presence of intracellular calcium ([Ca2+]i) oscillations. In mouse eggs, these oscillations cease after a variable period of time and this is accompanied by a decrease in inositol 1,4,5-trisphosphate receptor (IP3R) responsiveness and down-regulation of the IP3R type 1 (IP3R-1). To investigate the signaling pathway responsible for inducing IP3R-1 down-regulation during fertilization, mouse eggs were exposed to or injected with several Ca2+-releasing agonists and the amounts of IP3R-1 immunoreactivity evaluated by Western blotting. Exposure to ethanol or ionomycin, which induce a single [Ca2+]i rise, failed to signal down-regulation of IP3R-1. However, [Ca2+]i oscillations induced by injection of boar sperm fractions (SF), which presumably stimulate production of IP3, or adenophostin A, an IP3R agonist, both induced down-regulation of IP3R-1 of a magnitude similar to or greater than that observed after fertilization. Exposure to thimerosal, an oxidizing agent that modifies the IP3R without stimulating production of IP3, also initiated down-regulation ofIP3R-1, although oscillations initiated by SrCl2 failed to evoke down-regulation of IP3R-1. The degradation of IP3R-1 in mouse eggs appears to be mediated by the proteasome pathway because it was inhibited by preincubation with lactacystin, a very specific proteasome inhibitor. We therefore suggestthat persistent stimulation of the phosphoinositide pathway in mouse eggs by the sperm during fertilization or by injection of SF leads to down-regulation of the IP3R-1. (C) 2000 Academic Press.

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Documento generato il 28/11/20 alle ore 00:27:28