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Titolo:
Immunocytochemical detection of HSP70 in cell culture of pig granulosa
Autore:
Antalikova, L; Petr, J; Rozinek, J; Jilek, F;
Indirizzi:
Czech Univ Agr, Dept Vet Sci, Fak Agron, Prague 16521 6, Czech Republic Czech Univ Agr Prague Czech Republic 16521 6 gue 16521 6, Czech Republic Res Inst Anim Prod, Prague, Czech Republic Res Inst Anim Prod Prague Czech Republic m Prod, Prague, Czech Republic
Titolo Testata:
CZECH JOURNAL OF ANIMAL SCIENCE
fascicolo: 6, volume: 45, anno: 2000,
pagine: 249 - 255
SICI:
1212-1819(200006)45:6<249:IDOHIC>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEAT-SHOCK PROTEINS; MOLECULAR CHAPERONES; OOCYTE MATURATION; THERMOTOLERANCE; HYPERTHERMIA; CYTOSKELETON; INVITRO;
Keywords:
granulosa cells; culture; pig; HSP70; immunocytochemistry;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
22
Recensione:
Indirizzi per estratti:
Indirizzo: Rozinek, J Czech Univ Agr, Dept Vet Sci, Fak Agron, Kamycka 129, Prague 16521 6, Czech Republic Czech Univ Agr Kamycka 129 Prague Czech Republic 16521 6 ublic
Citazione:
L. Antalikova et al., "Immunocytochemical detection of HSP70 in cell culture of pig granulosa", CZEC J ANIM, 45(6), 2000, pp. 249-255

Abstract

The current study was undertaken to investigate the presence of heat shockproteins (HSPs) in a monolayer culture of pig ovarial granulosa cells submitted to stress conditions, and to exactly define heal shock (43 degrees C)during 1, 2, 4, and 6 hours. For the detection of HSPs we used immunocytochemical reaction with a monoclonal antiHSP70 antibody. The adherent granulosa cells growing in the monolayer showed the strongest reaction after 4 and6 hours' heat shock, especially when the culture was transported, after 6 hours of heat shock, into normal culture conditions (temperature of 37 degrees C). A very strong positive reaction was exceptionally concentrated within the nuclei. There was a substantially weak reaction within the cytoplasm. Reaction in the control culture cells was minimized and, after 1 hour of heat shock, the presence of HSPs was also very weak. The induction of HSPs into a culture of pig ovarial granulosa cells after heat shock presents a very interesting model for the study of cellular adaptive response to external stress.

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Documento generato il 29/11/20 alle ore 10:29:12