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Titolo:
Combined PI-DAPI staining (CPD) reveals NOR asymmetry and facilitates karyotyping of plant chromosomes
Autore:
Andras, SC; Hartman, TPV; Alexander, J; McBride, R; Marshall, JA; Power, JB; Cocking, EC; Davey, MR;
Indirizzi:
Univ Nottingham, Sch Biol Sci, Plant Sci Div, Nottingham NG7 2RD, England Univ Nottingham Nottingham England NG7 2RD , Nottingham NG7 2RD, England Univ Babes Bolyai, Fac Biol Geol, Dept Ecol & Genet, R-3400 Cluj Napoca, Romania Univ Babes Bolyai Cluj Napoca Romania R-3400 R-3400 Cluj Napoca, Romania Univ London Queen Mary & Westfield Coll, Sch Biol Sci, London E1 4NS, England Univ London Queen Mary & Westfield Coll London England E1 4NS S, England Appl Imaging Int Ltd, Biosci Ctr, Newcastle Upon Tyne NE1 4EP, Tyne & Wear, England Appl Imaging Int Ltd Newcastle Upon Tyne Tyne & Wear England NE14EP land
Titolo Testata:
CHROMOSOME RESEARCH
fascicolo: 5, volume: 8, anno: 2000,
pagine: 387 - 391
SICI:
0967-3849(2000)8:5<387:CPS(RN>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
IN-SITU HYBRIDIZATION; FISH; HETEROCHROMATIN; LOCALIZATION; BINDING; REGIONS; RICE;
Keywords:
chromosome banding; combined PI and DAPI (CPD); 4 ' 6-diamidino-2-phenylindol (DAPI); fluorescent staining; Lycopersicon; marker chromosome; nucleolar organiser region (NOR); Oryza; propidium iodide (PI);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
22
Recensione:
Indirizzi per estratti:
Indirizzo: Davey, MR Univ Nottingham, Sch Biol Sci, Plant Sci Div, Nottingham NG7 2RD, England Univ Nottingham Nottingham England NG7 2RD am NG7 2RD, England
Citazione:
S.C. Andras et al., "Combined PI-DAPI staining (CPD) reveals NOR asymmetry and facilitates karyotyping of plant chromosomes", CHROMOS RES, 8(5), 2000, pp. 387-391

Abstract

This paper presents a preparative and staining procedure for plant mitoticchromosomes that uses a combination of PI (propidium iodide) and DAPI (4',6-diamidino-2-phenylindol) and which reveals a pattern of high-affinity regions for these fluorochromes. Nucleolar organiser regions (NORs), telomeresand centromeric regions exhibit high PI affinity (red), whereas other chromosomal regions exhibit high affinity for either PI (red) or DAPI (blue). NOR-bearing and other chromosomes are readily distinguished, facilitating karyotyping. The dual staining pattern was observed in all the plants tested. Aspects of NOR size, number and occurrence are discussed. A karyotype of rice metaphase chromosomes is presented, based on their fluorescent banding patterns.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 00:27:51