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Titolo:
Analysis of the ligand-binding domain of macrophage colony-stimulating receptor
Autore:
Luo, SQ; Zheng, DX; Liu, YX; Rao, Q; Wu, KF;
Indirizzi:
Peking Union Med Coll, Chinese Acad Med Sci, Inst Hematol, State Key Lab Expt Hematol, Tianjin 300020, Peoples R China Peking Union Med Coll Tianjin Peoples R China 300020 20, Peoples R China Peking Union Med Coll, Chinese Acad Med Sci, Inst Basic Med Sci, State KeyLab Med Mol Biol, Beijing 100005, Peoples R China Peking Union Med Coll Beijing Peoples R China 100005 05, Peoples R China
Titolo Testata:
CHINESE SCIENCE BULLETIN
fascicolo: 13, volume: 45, anno: 2000,
pagine: 1191 - 1195
SICI:
1001-6538(200007)45:13<1191:AOTLDO>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
FACTOR-I RECEPTOR; AFFINITY;
Keywords:
M-CSF; binding ability; structure-function;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Physical, Chemical & Earth Sciences
Citazioni:
10
Recensione:
Indirizzi per estratti:
Indirizzo: Zheng, DX Peking Union Med Coll, Chinese Acad Med Sci, Inst Hematol, StateKey Lab Expt Hematol, Tianjin 300020, Peoples R China Peking Union Med Coll Tianjin Peoples R China 300020 s R China
Citazione:
S.Q. Luo et al., "Analysis of the ligand-binding domain of macrophage colony-stimulating receptor", CHIN SCI B, 45(13), 2000, pp. 1191-1195

Abstract

RNAs have been extracted from human placenta. Extracellular regions of M-CSFR, D1-3, D2-3 and D3 motifs have been amplified with RT-PCR. The proteinshave been expressed in E.coli. Enzyme-linked immusorbent assay (EIA) showsthat recombinant D1-3 possesses binding ability 3 times that of D2-3. Kd, the dissociation constant of the former is 11 nmol/L, and that of latter is39 nmol/L. D3 lacks binding ability. These data suggest that D2-3 is the main site for M-CSF binding, D1 is an assistant site and also contributes tothe conformation of site for ligand binding.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/11/20 alle ore 14:25:40