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Titolo:
Expression of fibrillins and other microfibril-associated proteins in human bone and osteoblast-like cells
Autore:
Kitahama, S; Gibson, MA; Hatzinikolas, G; Hay, S; Kuliwaba, JL; Evdokiou, A; Atkins, GJ; Findlay, DM;
Indirizzi:
Univ Adelaide, Dept Orthopaed & Trauma, Adelaide, SA 5000, Australia Univ Adelaide Adelaide SA Australia 5000 ma, Adelaide, SA 5000, Australia Univ Adelaide, Dept Pathol, Adelaide, SA 5000, Australia Univ Adelaide Adelaide SA Australia 5000 ol, Adelaide, SA 5000, Australia
Titolo Testata:
BONE
fascicolo: 1, volume: 27, anno: 2000,
pagine: 61 - 67
SICI:
8756-3282(200007)27:1<61:EOFAOM>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
ELASTIN-ASSOCIATED MICROFIBRILS; GROWTH-FACTOR-BETA; MARFAN-SYNDROME; EXTRACELLULAR-MATRIX; DEVELOPMENTAL EXPRESSION; GLYCOPROTEIN-1 MAGP-1; DERMAL FIBROBLASTS; MOLECULAR-CLONING; VI COLLAGEN; COMPONENT;
Keywords:
fibrillin; microfibril-associated proteins; human bone; osteoblast-like cells;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
46
Recensione:
Indirizzi per estratti:
Indirizzo: Findlay, DM Univ Adelaide, Dept Orthopaed & Trauma, Level 4,Bice Bldg, Adelaide, SA 5000, Australia Univ Adelaide Level 4,Bice Bldg Adelaide SA Australia 5000 lia
Citazione:
S. Kitahama et al., "Expression of fibrillins and other microfibril-associated proteins in human bone and osteoblast-like cells", BONE, 27(1), 2000, pp. 61-67

Abstract

Fibrillin-containing microfibrils are structural components of extracellular matrices of a diverse range of tissues, including bone. Their importancein bone biology is illustrated by the skeletal abnormalities manifest in the congenital disorder, Marfan syndrome, which results from mutations in the fibrillin-1 gene. We investigated the expression of fibrillins and other microfibril-associated proteins in human bone and bone-derived osteoblasts. Analysis of RNA extracted from cancellous bone showed expression of mRNAs encoding fibrillin-1 and -2, MAGP-1 and -2, LTBP-2, and MP78/70 (Big-h3), In demineralized normal mature bone, fibrillin-1 was immunolocalized to fibrils within the bone matrix and pericellularly to cells lining the endostealsurfaces of trabecular bone, some osteocytes, and cells associated with blood vessels. LTBP-2 was also identified at the endosteal surface and withinthe bone matrix in a lamellar fashion. In addition, primary osteoblast-like cells cultured from human trabecular bone (obtained from patients at joint replacement surgery) were found to express abundant mRNA for fibrillins and associated glycoproteins, Moreover, using western blot analysis, fibrillin-1 protein was shown to be secreted into the medium and to be deposited into the cell layer. Immunofluorescence staining of the cell layer visualized fibrillin-1 in the matrix as a three-dimensional network of fine filaments. Expression of fibrillin-1 by osteoblast-like cells was constitutive, anda number of skeletally active agents had little effect on mRNA or protein levels. These results show that human osteoblasts from mature bone express fibrillins and other microfibril-associated proteins, and suggest a role for these molecules in adult human bone. (Bone 27:61-67; 2000) (C) 2000 by Elsevier Science Inc, All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/07/20 alle ore 10:31:22