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Titolo:
Template-directed interference footprinting for RNA based on inosine-specific cleavage
Autore:
Jaikaran, DCJ; MacMillan, AM;
Indirizzi:
Univ Toronto, Dept Chem, Toronto, ON M5S 3HC, Canada Univ Toronto TorontoON Canada M5S 3HC Chem, Toronto, ON M5S 3HC, Canada
Titolo Testata:
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
fascicolo: 15, volume: 10, anno: 2000,
pagine: 1633 - 1635
SICI:
0960-894X(20000807)10:15<1633:TIFFRB>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
NUCLEOTIDE ANALOG INTERFERENCE; GROUP-I INTRON; TETRAHYMENA RIBOZYME; HAMMERHEAD RIBOZYME; BINDING-SITE; CONTACTS; RECOGNITION; CATALYSIS; CORE; DNA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: MacMillan, AM Univ Toronto, Dept Chem, 80 St George St, Toronto, ON M5S 3HC, Canada Univ Toronto 80 St George St Toronto ON Canada M5S 3HC anada
Citazione:
D.C.J. Jaikaran e A.M. MacMillan, "Template-directed interference footprinting for RNA based on inosine-specific cleavage", BIOORG MED, 10(15), 2000, pp. 1633-1635

Abstract

We report here the development of a Template-directed Interference (TDI) footprinting assay for RNA. The TDI nucleotide analogue inosine (I) lacks the exocyclic amine of G and is a suitable probe for the role of this group in RNA structure and function. Using an I-specific cleavage protocol we identified three functionally significant G residues in the Tetrahymena ribozyme. These residues are proximal to the active site of the folded intron and likely contribute to the positioning of substrates at the catalytic core. (C) 2000 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 15:23:34