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Titolo:
Novel responses of ZRF, a variant of human MTF-1, to in vivo treatment with heavy metals
Autore:
Otsuka, F; Okugaito, I; Ohsawa, M; Iwamatsu, A; Suzuki, K; Koizumi, S;
Indirizzi:
Teikyo Univ, Fac Pharmaceut Sci, Dept Environm Toxicol, Kanagawa 1990195, Japan Teikyo Univ Kanagawa Japan 1990195 ronm Toxicol, Kanagawa 1990195, Japan Kirin Brewery Co Ltd, Cent Labs Key Technol, Yokohama, Kanagawa 2360004, Japan Kirin Brewery Co Ltd Yokohama Kanagawa Japan 2360004 agawa 2360004, Japan Natl Inst Ind Hlth, Div Hazard Assessment, Kawasaki, Kanagawa 2148585, Japan Natl Inst Ind Hlth Kawasaki Kanagawa Japan 2148585 anagawa 2148585, Japan
Titolo Testata:
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION
fascicolo: 2-3, volume: 1492, anno: 2000,
pagine: 330 - 340
SICI:
0167-4781(20000724)1492:2-3<330:NROZAV>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANSCRIPTION FACTOR MTF-1; METALLOTHIONEIN-IIA GENE; REGULATORY ELEMENTS; NUCLEAR-PROTEIN; DNA-BINDING; FACTOR-I; ZINC; INTERACTS; CLONING; PURIFICATION;
Keywords:
metallothionein; MTF-1; metal responsive element; heavy metal; transcription factor; cloning;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Otsuka, F Teikyo Univ, Fac Pharmaceut Sci, Dept Environm Toxicol, Kanagawa1990195, Japan Teikyo Univ Kanagawa Japan 1990195 ol, Kanagawa 1990195, Japan
Citazione:
F. Otsuka et al., "Novel responses of ZRF, a variant of human MTF-1, to in vivo treatment with heavy metals", BBA-GENE ST, 1492(2-3), 2000, pp. 330-340

Abstract

Heavy metal-dependent transcriptional activation of metallothionein (MT) genes is mediated by multiple enhancer sequences. metal responsive element (MRE), located in the upstream region of the genes. Previously, we have reported purification of a zinc-dependent MRE-binding protein, zinc regulatory factor (ZRF), from HeLa cells, and have pointed to the close relationship between ZRF and mouse MRE-binding transcription factor-1 (MTF-1) according to the analysis of partial amino acid sequences. By means of cDNA cloning and the product analyses, we show that ZRF is a variant of human MTF-1 (hMTF-1). which carries a single amino acid exchange in the zinc finger domain. Accordingly, ZRF is renamed hMTF-1b. Expression of hMTF-1b in HeLa cells is constitutive at both mRNA and protein levels, and is unaffected by treatment with cadmium (Cd). On the other hand, when cells were fractionated into nuclear extract and cytosol, a large part of the hMTF-1b was recovered in the cytosol fraction. A significant increase in the amount of nuclear hMTF-1boccurs when cells are treated with various heavy metals, including Cd, Zn,Cu and Ag, which is associated with concomitant decrease in the amount recovered in the cytosol fraction. Since immunocytochemical analysis revealed that intracellular distribution of hMTF-1b is restricted to the nucleus irrespective of the heavy metal treatment, such an increment in the nuclear extracts apparently results from promotion of nuclear retention of hMTF-1b bythe heavy metal treatment. Analysis by native gel electrophoresis shows that the mobility of hMTF-1b significantly changes in association with Cd treatment, raising the possibility that a conformational change of hMTF-1b occurs in response to treatment with heavy metals in vivo. (C) 2000 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/21 alle ore 10:07:19