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Titolo:
Detection and quantification of neurotensin in human brain tissue by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
Autore:
Gobom, J; Kraeuter, KO; Persson, R; Steen, H; Roepstorff, P; Ekman, R;
Indirizzi:
Univ Gothenburg, Neurochem Sect, Inst Clin Neurosci, SU Moelndal Hosp, S-43180 Molndal, Sweden Univ Gothenburg Molndal Sweden S-43180 dal Hosp, S-43180 Molndal, Sweden Univ So Denmark, Prot Res Grp, Dept Mol Biol, Odense, Denmark Univ So Denmark Odense Denmark Res Grp, Dept Mol Biol, Odense, Denmark Bruker Dalton, Bremen, Germany Bruker Dalton Bremen GermanyBruker Dalton, Bremen, Germany Univ So Denmark, Prot Interact Lab, Dept Mol Biol, Odense, Denmark Univ SoDenmark Odense Denmark ract Lab, Dept Mol Biol, Odense, Denmark
Titolo Testata:
ANALYTICAL CHEMISTRY
fascicolo: 14, volume: 72, anno: 2000,
pagine: 3320 - 3326
SICI:
0003-2700(20000715)72:14<3320:DAQONI>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
ELECTROSPRAY SAMPLE PREPARATION; DESORPTION IONIZATION; QUANTITATIVE-ANALYSIS; FAST-EVAPORATION; CYCLOSPORINE-A; NEUROMEDIN-N; PEPTIDE; REPRODUCIBILITY; PROTEIN; CHROMATOGRAPHY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Physical, Chemical & Earth Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Ekman, R Univ Gothenburg, Neurochem Sect, Inst Clin Neurosci, SU Moelndal Hosp, S-43180 Molndal, Sweden Univ Gothenburg Molndal Sweden S-43180 S-43180 Molndal, Sweden
Citazione:
J. Gobom et al., "Detection and quantification of neurotensin in human brain tissue by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry", ANALYT CHEM, 72(14), 2000, pp. 3320-3326

Abstract

A method was developed for mass spectrometric detection of neurotensin (NT)-like immunoreactivity and quantification of NT in human brain tissue. Themethod is based on immunoprecipitation followed by analysis using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS), The identity of the major component of the immunoprecipitates as neurotensin was confirmed by fragment ion analysis on an electrospray ionization quadrupole time-of-flight instrument. MALDI-TOF-MS quantification of NT was achieved using stableisotope-labeled NT as the internal standard, yielding an error of less than 5%, The method allowed detection of low-femtomole amounts of NT, starting from low-milligram amounts of lyophilized braintissue. In addition to NT, several other peptides were detected in the purified samples, most of which, according to their molecular masses, corresponded to fragments of NT, The method is demonstrated with quantification of NT from human hypothalamus tissue, and a comparison is made with results obtained from competitive radioimmunoassay.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 06:58:53