Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Clathrin in mitotic spindles
Autore:
Okamoto, CT; McKinney, J; Jeng, YY;
Indirizzi:
Univ So Calif, Sch Pharm, Dept Pharmaceut Sci, Los Angeles, CA 90089 USA Univ So Calif Los Angeles CA USA 90089 eut Sci, Los Angeles, CA 90089 USA
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
fascicolo: 2, volume: 279, anno: 2000,
pagine: C369 - C374
SICI:
0363-6143(200008)279:2<C369:CIMS>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
AP-3 ADAPTER COMPLEX; COATED VESICLES; GOLGI MEMBRANES; 100-KD PROTEINS; BREFELDIN-A; CELLS; BINDING; RECRUITMENT; PATHWAY; SITES;
Keywords:
Madin-Darby canine kidney cells; CV-1 cells; brefeldin A;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Okamoto, CT Univ So Calif, Sch Pharm, Dept Pharmaceut Sci, Los Angeles, CA90089 USA Univ So Calif Los Angeles CA USA 90089 Angeles, CA 90089 USA
Citazione:
C.T. Okamoto et al., "Clathrin in mitotic spindles", AM J P-CELL, 279(2), 2000, pp. C369-C374

Abstract

Subconfluent cultures of Madin-Darby canine kidney (MDCK) and CV-1 cells were immunostained with two monoclonal antibodies (MAbs), MAb X-22 and MAb 23, against clathrin heavy chain and with polyclonal antiserum against a conserved region of all mammalian clathrin light chains. In interphase MDCK and CV-1 cells, staining by all three antibodies resulted in the characteristic intracellular punctate vesicular and perinuclear staining pattern. In mitotic cells, all three anticlathrin antibodies strongly stained the mitoticspindle. Staining of clathrin in the mitotic spindle was colocalized with anti-tubulin staining of microtubular arrays in the spindle. Staining of the mitotic spindle was evident in mitotic cells from prometaphase to telophase and in spindles in mitotic cells released from a thymidine-nocodazole block. In CV-1 cells, staining of clathrin in the mitotic spindle was not affected by brefeldin A. On Western blots, clathrin was detected, but not enriched, in isolated spindles. The immunodetection of clathrin in the mitotic spindle may suggest a novel role for clathrin in mitosis. Alternatively, the recruitment of clathrin to the spindle may suggest a novel regulatory mechanism for localization of clathrin in mitotic cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 06:09:12