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Titolo:
Presence of a higher molecular weight beta-1,4-galactosyltransferase in mouse liver
Autore:
Kido, M; Asano, M; Iwakura, Y; Ichinose, M; Miki, K; Furukawa, K;
Indirizzi:
Tokyo Metropolitan Inst Gerontol, Dept Biosignal Res, Itabashi Ku, Tokyo 1730015, Japan Tokyo Metropolitan Inst Gerontol Tokyo Japan 1730015 okyo 1730015, Japan Univ Tokyo, Fac Med, Dept Gastroenterol, Bunkyo Ku, Tokyo 1138655, Japan Univ Tokyo Tokyo Japan 1138655 oenterol, Bunkyo Ku, Tokyo 1138655, Japan Univ Tokyo, Inst Med Sci, Ctr Med Expt, Minato Ku, Tokyo 1080071, Japan Univ Tokyo Tokyo Japan 1080071 Med Expt, Minato Ku, Tokyo 1080071, Japan Toho Univ, Sch Med, Dept Internal Med 1, Ota Ku, Tokyo 1438541, Japan TohoUniv Tokyo Japan 1438541 ternal Med 1, Ota Ku, Tokyo 1438541, Japan
Titolo Testata:
ACTA HISTOCHEMICA ET CYTOCHEMICA
fascicolo: 3, volume: 33, anno: 2000,
pagine: 215 - 221
SICI:
0044-5991(2000)33:3<215:POAHMW>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
BETA-4-GALACTOSYLTRANSFERASE GENE FAMILY; UDP-GALACTOSE; GALACTOSYLTRANSFERASE ISOENZYME; CLONING; PURIFICATION; SPECIFICITY; EXPRESSION; CELLS; GLYCOPROTEINS; CARBOHYDRATE;
Keywords:
beta-1,4-salactosyltransferases; high molecular weight; N-linked oligosaccharides; mouse liver;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Furukawa, K Tokyo Metropolitan Inst Gerontol, Dept Biosignal Res, ItabashiKu, Tokyo 1730015, Japan Tokyo Metropolitan Inst Gerontol Tokyo Japan 1730015 , Japan
Citazione:
M. Kido et al., "Presence of a higher molecular weight beta-1,4-galactosyltransferase in mouse liver", ACT HIST CY, 33(3), 2000, pp. 215-221

Abstract

Histochemical analyses of beta-1,4-galactosyl-transferase (beta-1,4-GalT) I-knockout mouse liver using anti-bovine beta-1,4-GalT I antibody and Ricinus communis agglutinin (RCA)-I which interacts with oligosaccharides terminated with beta-1,4-linked galactose revealed that weak fluorescence derivedfrom the lectin is present in the mutant mouse in contrast to the disappearance of the immunofluorescence. Lectin blot analysis of membrane glycoproteins showed that many protein bands react with RCA-I although their reactivities are weak in the mutant mouse liver. Upon pretreatment of blots with beta-1,4-galactosidase or N-glycanase, no lectin binding was observed, indicating that a part of N-linked oligosaccharides of beta-1,4-GalT I-knockout mouse liver is galactosylated by yet unidentified beta-1,4-GalT. Gel filtration column chromatography of solubilized mouse liver transferases revealedthat beta-1,4-GalT activities in wild-type mouse are detected in fractions42 and 50 while those in the mutant mouse are detected only in fraction 42. The results indicate that mouse liver contains a high molecular weight beta-1,4-GalT (110 K) in addition to a previously known beta-1,4-GalT (46 K).

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Documento generato il 26/01/21 alle ore 04:22:11