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Titolo:
Mutations occurring during serial passage of Japanese equine infectious anemia virus in primary horse macrophages
Autore:
Zheng, YH; Sentsui, H; Kono, Y; Ikuta, K;
Indirizzi:
Hokkaido Univ, Inst Immunol Sci, Kita Ku, Sapporo, Hokkaido 060, Japan Hokkaido Univ Sapporo Hokkaido Japan 060 Ku, Sapporo, Hokkaido 060, Japan Natl Inst Anim Hlth, Tsukuba, Ibaraki 305, Japan Natl Inst Anim Hlth Tsukuba Ibaraki Japan 305 Tsukuba, Ibaraki 305, Japan Tokyo Univ Agr & Technol, Fac Agr, Tokyo 156, Japan Tokyo Univ Agr & Technol Tokyo Japan 156 hnol, Fac Agr, Tokyo 156, Japan Osaka Univ, Microbial Dis Res Inst, Dept Virol, Suita, Osaka 5650871, Japan Osaka Univ Suita Osaka Japan 5650871 t Virol, Suita, Osaka 5650871, Japan
Titolo Testata:
VIRUS RESEARCH
fascicolo: 1, volume: 68, anno: 2000,
pagine: 93 - 98
SICI:
0168-1702(200006)68:1<93:MODSPO>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
REPLICATION IN-VITRO; FEBRILE EPISODES; REGION; GENE; VIVO; DNA;
Keywords:
mutations; anemia; macrophages;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
17
Recensione:
Indirizzi per estratti:
Indirizzo: Ikuta, K Hokkaido Univ, Inst Immunol Sci, Kita Ku, Sapporo, Hokkaido 060, Japan Hokkaido Univ Sapporo Hokkaido Japan 060 ro, Hokkaido 060, Japan
Citazione:
Y.H. Zheng et al., "Mutations occurring during serial passage of Japanese equine infectious anemia virus in primary horse macrophages", VIRUS RES, 68(1), 2000, pp. 93-98

Abstract

An attenuated equine infectious anemia virus (EIAV), named V26, was previously obtained after 50 passages of the Japanese virulent strain V70 in primary macrophage culture. To clarify the differences between both viruses, their full-length sequences were determined. There were higher mutations in S2 (6.15% amino acid difference) and LTR (10.7% nucleotide difference). The presumed initiation codon of the S2 gene was absent from the sequence of V26. There was a large insertion within the long-terminal repeat (LTR) U3 hypervariable region of V26. In addition, there were minor mutations in gag (1.22% amino acid difference), pol (1.05% amino acid difference) and env (1.65% amino acid difference) regions, but no mutation in tnt region. No mutations were observed in the principal neutralizing domain in the gp90. Thus, the mutations in the S2 and LTR might be the major target sites of mutation in EIAV during serial passages in vitro. (C) 2000 Elsevier Science B.V. Allrights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/01/20 alle ore 14:55:00