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Titolo:
Direct measurement of CD8+T cell responses in macaques infected with simian immunodeficiency virus
Autore:
Donahoe, SM; Moretto, WJ; Samuel, RV; Metzner, KJ; Marx, PA; Hanke, T; Connor, RI; Nixon, DF;
Indirizzi:
Rockefeller Univ, Aaron Diamond AIDS Res Ctr, New York, NY 10016 USA Rockefeller Univ New York NY USA 10016 DS Res Ctr, New York, NY 10016 USA John Radcliffe Hosp, Inst Mol Med, MRC, Human Immunol Unit, Oxford OX3 9DS, England John Radcliffe Hosp Oxford England OX3 9DS Unit, Oxford OX3 9DS, England Tulane Univ, Med Ctr, Dept Trop Med, Covington, LA 70433 USA Tulane Univ Covington LA USA 70433 Dept Trop Med, Covington, LA 70433 USA Tulane Univ, Med Ctr, Tulane Reg Primate Res Ctr, Covington, LA 70433 USA Tulane Univ Covington LA USA 70433 imate Res Ctr, Covington, LA 70433 USA
Titolo Testata:
VIROLOGY
fascicolo: 2, volume: 272, anno: 2000,
pagine: 347 - 356
SICI:
0042-6822(20000705)272:2<347:DMOCCR>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
CYTOTOXIC T-LYMPHOCYTES; COMBINATION ANTIRETROVIRAL THERAPY; I-PEPTIDE COMPLEX; RHESUS-MONKEYS; FLOW-CYTOMETRY; PERIPHERAL-BLOOD; HIV-1 INFECTION; CHILDREN BORN; CTL EPITOPE; INDIVIDUALS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Nixon, DF Rockefeller Univ, Aaron Diamond AIDS Res Ctr, 455 1st Ave, New York, NY 10016 USA Rockefeller Univ 455 1st Ave New York NY USA 10016 NY 10016 USA
Citazione:
S.M. Donahoe et al., "Direct measurement of CD8+T cell responses in macaques infected with simian immunodeficiency virus", VIROLOGY, 272(2), 2000, pp. 347-356

Abstract

The simian immunodeficiency virus (SIV) macaque model system has been usedextensively to study AIDS pathogenesis and to test candidate vaccines for their ability to protect against homologous or heterologous challenge with pathogenic SIV or SHIV. Recent studies suggest that stimulation of HIV-1-specific CTL responses is important for effective vaccination against HIV-1. While quantitative measurements of SIV-specific cytotoxic T lymphocyte (CTL) responses have been facilitated by the use of tetrameric peptide complexes, this technique is currently limited to the study of Mamu-A*01-positive rhesus macaques. Furthermore, very few SIV-specific CTL epitopes have been identified, and there is limited identification of other MHC alleles in macaques. in this study, cytokine flow cytometry (CFC) was used to quantify SIV-specific CD8+ antigen-reactive T cells in macaques infected with SIV. We found a strong correlation (r = 0.96, P < 0.001) between CD8+ antigen-reactive T cells stained with the Mamu-A*01 p11C, C-M tetramer and production of intracellular TNF-or in the CFC assay. Furthermore, the CFC assay was used to identify a novel SIV-specific CTL epitope in Envelope (SIV Env, a.a. 486-494, sequence AEVAELYRL). The use of the CFC assay facilitates the study of antigen-reactive T cell responses in SIV infection and vaccination. (C) 2000 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/09/20 alle ore 07:46:08