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Titolo:
Detection of DNA segment utilizing an immobilized DNA electrode and an osmium complex
Autore:
Mishima, Y; Motonaka, J; Maruyama, K; Minagawa, K; Ikeda, S;
Indirizzi:
Univ Tokushima, Fac Engn, Dept Chem Sci & Technol, Tokushima 7708506, Japan Univ Tokushima Tokushima Japan 7708506 Technol, Tokushima 7708506, Japan
Titolo Testata:
SENSORS AND ACTUATORS B-CHEMICAL
fascicolo: 1-3, volume: 65, anno: 2000,
pagine: 340 - 342
SICI:
0925-4005(20000630)65:1-3<340:DODSUA>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Keywords:
immobilized DNA electrode; recognition of DNA sequences; osmium complex;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Physical, Chemical & Earth Sciences
Engineering, Computing & Technology
Citazioni:
7
Recensione:
Indirizzi per estratti:
Indirizzo: Mishima, Y Univ Tokushima, Fac Engn, Dept Chem Sci & Technol, Minamijosanjima 2-1, Tokushima 7708506, Japan Univ Tokushima Minamijosanjima 2-1 Tokushima Japan 7708506 pan
Citazione:
Y. Mishima et al., "Detection of DNA segment utilizing an immobilized DNA electrode and an osmium complex", SENS ACTU-B, 65(1-3), 2000, pp. 340-342

Abstract

The interactions of an osmium complex with immobilized dsDNA and ssDNA electrodes were studied by linear sweep voltammetry. The appearance of an oxidation peak of the complex indicated that the osmium complex specifically interacted with dsDNA. The osmium complex was concluded to interact electrostatically with phosphate groups, and nonelectrostatically with base pairs and/or the sugar backbone. Recognition of DNA sequences by the osmium complexwas examined. The immobilized ssDNA electrode was hybridized with probes, and then the charge of DNA hybrids were measured in phosphate buffer (pH 7.0). After interaction of this electrode with the osmium complex, the chargeof the complex was measured in phosphate buffer (pH 7.0). Results showed that complementary probes of more than 12 mers were recognized by the osmiumcomplex, and so that the osmium complex binds to major or minor grooves ofthe DNA helix. (C) 2000 Elsevier Science S.A. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 12:21:27