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Titolo:
Stimulation of mitotic recombination events by high levels of RNA polymerase II transcription in yeast
Autore:
Saxe, D; Datta, A; Jinks-Robertson, S;
Indirizzi:
Emory Univ, Dept Biol, Atlanta, GA 30322 USA Emory Univ Atlanta GA USA 30322 ry Univ, Dept Biol, Atlanta, GA 30322 USA Emory Univ, Grad Program Genet & Mol Biol, Atlanta, GA 30322 USA Emory Univ Atlanta GA USA 30322 m Genet & Mol Biol, Atlanta, GA 30322 USA Emory Univ, Grad Program Biochem & Mol Biol, Atlanta, GA 30322 USA Emory Univ Atlanta GA USA 30322 Biochem & Mol Biol, Atlanta, GA 30322 USA
Titolo Testata:
MOLECULAR AND CELLULAR BIOLOGY
fascicolo: 15, volume: 20, anno: 2000,
pagine: 5404 - 5414
SICI:
0270-7306(200008)20:15<5404:SOMREB>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
SACCHAROMYCES-CEREVISIAE; MEIOTIC RECOMBINATION; S-CEREVISIAE; HOMOLOGOUS RECOMBINATION; ECTOPIC RECOMBINATION; ESCHERICHIA-COLI; MAMMALIAN-CELLS; EXCISION-REPAIR; GENE CONVERSION; RIBOSOMAL DNA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
51
Recensione:
Indirizzi per estratti:
Indirizzo: Jinks-Robertson, S Emory Univ, Dept Biol, 1510 Clifton Rd, Atlanta, GA 30322 USA Emory Univ 1510 Clifton Rd Atlanta GA USA 30322 322 USA
Citazione:
D. Saxe et al., "Stimulation of mitotic recombination events by high levels of RNA polymerase II transcription in yeast", MOL CELL B, 20(15), 2000, pp. 5404-5414

Abstract

The impact of high levels of RNA polymerase II transcription on mitotic recornbination was examined using lys2 recombination substrates positioned onnonhomologous chromosomes. Substrates were used that could produce Lys(+) recombinants by either a simple (noncrossover) gene conversion event or a crossover-associated recombination event, by only a simple gene conversion event, or by only a crossover event. Transcription of the lys2 substrates was regulated by the highly inducible GAL1-10 promoter or the low-level LYS2 promoter, with GAL1-10 promoter activity being controlled by the presence or absence of the Gal80p negative regulatory protein. Transcription was found to stimulate recombination in all assays used, but the level of stimulation varied depending on whether only one or both substrates were highly transcribed. In addition, there was an asymmetry in the types of recombination events observed when one substrate versus the other was highly transcribed. Finally, the lys2 substrates were positioned as direct repeats on the samechromosome and were found to exhibit a different recombinational response to high levels of transcription from that exhibited by the repeats on nonhomologous chromosomes. The relevance of these results to the mechanisms of transcription-associated recombination are discussed.

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Documento generato il 14/07/20 alle ore 08:08:58