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Titolo:
Influence of sustained mechanical stress on Egr-1 mRNA expression in cultured human endothelial cells
Autore:
Stula, M; Orzechowski, HD; Gschwend, S; Vetter, R; von Harsdorf, R; Dietz, R; Paul, M;
Indirizzi:
Free Univ Berlin, Benjamin Franklin Med Ctr, Inst Clin Pharmacol & Toxicol, D-14195 Berlin, Germany Free Univ Berlin Berlin Germany D-14195 Toxicol, D-14195 Berlin, Germany Humboldt Univ, Franz Volhard Klin, Dept Mol & Clin Cardiol, Berlin, Germany Humboldt Univ Berlin Germany , Dept Mol & Clin Cardiol, Berlin, Germany
Titolo Testata:
MOLECULAR AND CELLULAR BIOCHEMISTRY
fascicolo: 1-2, volume: 210, anno: 2000,
pagine: 101 - 108
SICI:
0300-8177(200007)210:1-2<101:IOSMSO>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACTIVATED ION CHANNELS; SIGNAL-TRANSDUCTION PATHWAYS; SERUM RESPONSE ELEMENTS; FLUID SHEAR-STRESS; SMOOTH-MUSCLE CELL; MYOCYTES IN-VITRO; A-CHAIN PROMOTER; GENE-EXPRESSION; CARDIAC MYOCYTES; C-FOS;
Keywords:
human endothelial cells; mechanical stress; immediate early genes; protein kinase C; calcium;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Paul, M Free Univ Berlin, Benjamin Franklin Med Ctr, Inst Clin Pharmacol &Toxicol, Garystr 5, D-14195 Berlin, Germany Free Univ Berlin Garystr 5 Berlin Germany D-14195 Berlin, Germany
Citazione:
M. Stula et al., "Influence of sustained mechanical stress on Egr-1 mRNA expression in cultured human endothelial cells", MOL C BIOCH, 210(1-2), 2000, pp. 101-108

Abstract

Restenosis after initially successful balloon angioplasty of coronary artery stenosis remains a major problem in clinical cardiology. Previous studies have identified pathogenetic factors which trigger cell proliferation andvascular remodeling ultimately leading to restenosis. Since there is evidence that endothelial cells adjacent to the angioplasty wound area synthesize factors which may initiate this process, we investigated the effects of mechanical stimulation on endothelial gene expression in vitro and focussed on the influence of sustained mechanical stress on expression of immediate early genes which have previously been shown to be induced in the vascular wall in vivo. Primary cultured human umbilical vein endothelial cells (HUVEC) and the human endothelial cell line EA.hy 926 were plated on collagen-coated silicone membranes and subjected to constant longitudinal stress of approximately 20% for 10 min to 6 h. Total RNA was isolated and the expression of the immediate early genes c-Fos and Egr-1 was studied by Northern blotanalysis. We found a rapid upregulation c-Fos and Egr-1 mRNA which startedat 10 min and reached its maxima at 30 min. HUVEC lost most of their stretch response after the third passage whereas immediate early gene expressionwas constantly in EA.hy 926 cells. Using specific inhibitors we investigated the contribution of several signal transduction pathways to stretch-activated Egr-1 mRNA expression. We found significant suppression of stretch-induced Egr-1 mRNA expression by protein kinase C (PKC) inhibition (p < 0.05)and by calcium depletion (EA.hy926, p < 0.05; HUVEC, p = 0.063). No effecton stretch-activated Egr-1 mRNA expression was detected by inhibition of protein kinase A, blockade of stretch-activated cation channels or inhibition of microtubule synthesis. We conclude that sustained mechanical strain induces Egr-1 mRNA expression by PKC- and calcium-dependent mechanisms.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 10:31:15