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Titolo:
Modulation of cAMP level by tedisamil in guinea pig heart
Autore:
Tribulova, N; Ravingerova, T; Okruhlicova, L; Gabauer, I; Fickova, M; Pancza, D; Slezak, J; Manoach, M;
Indirizzi:
Slovak Acad Sci, Heart Res Inst, Bratislava 84233, Slovakia Slovak Acad Sci Bratislava Slovakia 84233 st, Bratislava 84233, Slovakia Slovak Acad Sci, Inst Endocrinol, Bratislava 84233, Slovakia Slovak Acad Sci Bratislava Slovakia 84233 ol, Bratislava 84233, Slovakia Sackler Sch Med, Dept Physiol, Tel Aviv, Israel Sackler Sch Med Tel AvivIsrael Sch Med, Dept Physiol, Tel Aviv, Israel
Titolo Testata:
MOLECULAR AND CELLULAR BIOCHEMISTRY
fascicolo: 1-2, volume: 210, anno: 2000,
pagine: 75 - 80
SICI:
0300-8177(200007)210:1-2<75:MOCLBT>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
CYCLIC ADENOSINE-MONOPHOSPHATE; VENTRICULAR-FIBRILLATION; MYOCARDIAL-INFARCTION; CARDIAC MYOCYTES; ISCHEMIA; COMMUNICATION; CONTRACTILITY; CONDUCTANCE; ARRHYTHMIAS; CALCIUM;
Keywords:
tedisamil; guinea pig heart; cAMP; ischemia;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Tribulova, N Slovak Acad Sci, Heart Res Inst, Dubravska Cesta 9, Bratislava 84233, Slovakia Slovak Acad Sci Dubravska Cesta 9 Bratislava Slovakia 84233
Citazione:
N. Tribulova et al., "Modulation of cAMP level by tedisamil in guinea pig heart", MOL C BIOCH, 210(1-2), 2000, pp. 75-80

Abstract

Tedisamil is antiarrhythmic class III drug with antifibrillating/defibrillating potency linked to enhancement of intermyocyte gap junctional electrical coupling most likely via its sympathomimetic cAMP-related mechanisms. This study was designed to examin the effect of tedisamil on cAMP level in guinea pig hearts in vivo and in vitro in Langendorff preparation. The drug was administered either as a bolus into vena jugularis in dosage 1.0 and 1.5mg/kg or into the perfusion solution at a concentration of 1.5 x 10(-6) mol/l. In additional experiments, this period was followed by brief 10 min global ischemia, induced by clamping of the aorta or perfusion. After 10 min from the onset of tedisamil administration as well as after 10 min of ischemia the ventricular tissue was immediately frozen for cAMP immunoassay Tedisamil caused in normal heart small but significant dose-dependent increase of myocardial cAMP (pmol/mg) level in vivo 1.8 and 2.5 vs. 1.4 as well as in vitro 1.1 vs. 0.8 (p < 0.05) conditions. Ischemia itself induced accumulation of cAMP in both, in vitro and in vitro experiments, 2.6 vs. 1.4 and 1.3 vs. 0.8, respectively. The preischemic elevation of cAMP by tedisamil wasnot potentiated by following ischemia, on the contrary, decline of the cyclic nucleotide was detected comparing to ischemia itself. In conclusion, tedisamil increased cAMP level in normal heart and prevented additional ischemia-related elevation of this nucleotide. The results indicate modulation of myocardial cAMP level by tedisamil, which may account for its protective effect on gap junctional electrical coupling.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/09/20 alle ore 22:52:09