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Titolo:
Image analysis and flow cytometric DNA studies of benign and malignant body cavity fluids: Reappraisal of the role of current methods in the differential diagnosis of reactive versus malignant conditions
Autore:
Lazcano, O; Chen, LM; Tsai, C; Li, CY; Katzmann, JA; Sebo, TJ; Kimlinger, TK; Baker, J;
Indirizzi:
Mayo Clin & Mayo Fdn, Dept Lab Med & Pathol, Rochester, MN 55905 USA Mayo Clin & Mayo Fdn Rochester MN USA 55905 thol, Rochester, MN 55905 USA Forest Pk Hosp, Immunocytometr Lab, Dept Pathol, St Louis, MO USA Forest Pk Hosp St Louis MO USA tometr Lab, Dept Pathol, St Louis, MO USA
Titolo Testata:
MODERN PATHOLOGY
fascicolo: 7, volume: 13, anno: 2000,
pagine: 788 - 796
SICI:
0893-3952(200007)13:7<788:IAAFCD>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
PLEURAL MESOTHELIOMAS; PROGNOSTIC-SIGNIFICANCE; MONOCLONAL-ANTIBODIES; LUNG ADENOCARCINOMAS; PLOIDY ANALYSIS; EFFUSIONS; IMMUNOCYTOCHEMISTRY; ANEUPLOIDY; CYTOLOGY; MARKER;
Keywords:
body cavity fluids; cytology; DNA ploidy; flow cytometry; image analysis; mesothelial cells;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Li, CY Mayo Clin & Mayo Fdn, Dept Lab Med & Pathol, 200 1st St SW, Rochester, MN 55905 USA Mayo Clin & Mayo Fdn 200 1st St SW Rochester MN USA 55905 55905 USA
Citazione:
O. Lazcano et al., "Image analysis and flow cytometric DNA studies of benign and malignant body cavity fluids: Reappraisal of the role of current methods in the differential diagnosis of reactive versus malignant conditions", MOD PATHOL, 13(7), 2000, pp. 788-796

Abstract

Cytologic examination of body fluids is commonly performed in the clinicallaboratory. Determination of the presence of malignancy may sometimes be difficult. In this study, we prospectively studied 60 body fluids with a panel of antibodies, including MOC-31, epithelial membrane antigen, carcinoembryonic antigen, B72.3, keratin, desmin, and CA-125. DNA and S-phase studieswere performed both by now cytometry and image analysis. Thirty-seven fluids were classified as benign and 23 were classified as malignant. The sensitivity of the antibodies for identification of carcinoma in descending order of percentage detection rate were MOC-31 (95%), epithelial membrane antigen (93%), B72.3 (84%), and carcinoembryonic antigen (80%). Desmin stained mesothelial cells in all cases. CA-125 gave similar results but was less specific. Flow cytometry detected 14 of 20 malignant fluids and image analysis17 of 23 by identifying an aneuploid population. Benign reactive mesothelial cells were not aneuploid. Tetraploidy due to reactive mesothelial cells was found in 9 of 37 body fluids. Their S-phase fraction was low (average, 3.2%). Tetraploidy in malignant cells was distinguished from the reactive mesothelial cells by high S-phase (average, 25.95). S-phase had some use as a discriminating factor, because no benign reactive cases had more than 17%. However, 7 of 23 malignant cases had a value below 17%. DNA analysis by image was more sensitive the and specific than now. Either may be used when immunocytochemistry is nondiagnostic or cannot be performed.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 20:01:48