Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
THE RAT D-4 DOPAMINE-RECEPTOR COUPLES TO CONE TRANSDUCIN (G-ALPHA(T2)) TO INHIBIT FORSKOLIN-STIMULATED CAMP ACCUMULATION
Autore:
YAMAGUCHI I; HARMON SK; TODD RD; OMALLEY KL;
Indirizzi:
WASHINGTON UNIV,SCH MED,DEPT ANAT & NEUROBIOL,BOX 8108,660 S EUCLID AVE ST LOUIS MO 63110 WASHINGTON UNIV,SCH MED,DEPT ANAT & NEUROBIOL ST LOUIS MO 63110 WASHINGTON UNIV,SCH MED,DEPT PSYCHIAT ST LOUIS MO 63110 WASHINGTON UNIV,SCH MED,DEPT GENET ST LOUIS MO 63110
Titolo Testata:
The Journal of biological chemistry
fascicolo: 26, volume: 272, anno: 1997,
pagine: 16599 - 16602
SICI:
0021-9258(1997)272:26<16599:TRDDCT>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
FUNCTIONAL-CHARACTERIZATION; MOLECULAR-CLONING; ALPHA-SUBUNIT; EXPRESSION; TRANSDUCIN; CELLS; GENE; SEQUENCE; PHOTORECEPTORS; AFFINITY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
36
Recensione:
Indirizzi per estratti:
Citazione:
I. Yamaguchi et al., "THE RAT D-4 DOPAMINE-RECEPTOR COUPLES TO CONE TRANSDUCIN (G-ALPHA(T2)) TO INHIBIT FORSKOLIN-STIMULATED CAMP ACCUMULATION", The Journal of biological chemistry, 272(26), 1997, pp. 16599-16602

Abstract

Based on its expression pattern and pharmacology, the D-4 dopamine receptor may play a role in schizophrenia. Thus it is of interest to know what signaling pathways are utilized by this receptor. Previously, we showed that activation of D-4 receptors in a mouse mesencephalic neuronal cell line (MN9D) inhibited forskolin-stimulated cAMP accumulation in a pertussis toxin-sensitive (Ptx-sensitive) fashion. Of the knownPtx-sensitive G-protein alpha subunits, MN9D expressed G alpha(i2), Galpha(oA), and G alpha(oB); however, none of these coupled td the D-4receptor. Using a low stringency polymerase chain reaction cloning method, we found an additional Ptx-sensitive G-protein cone transducin (G alpha(t2)) expressed in the MN9D cells. We also found that G alpha(t2) mRNA is highly expressed in rat mesencephalic tissue. To test the hypothesis that the D-4 receptor couples to G alpha(t2) we cotransfected MNSD cells with the D-4 receptor and a mutagenized Ptx-resistant G alpha(t2) subunit (mG alpha(t2)). Application of the dopaminergic agonist quinpirole to cotransfected cells inhibited forskolin-stimulated cAMP accumulation in the presence or absence of Ptx. To our knowledge, this is the first report demonstrating that the D-4 dopamine receptor functionally couples to a specific G-protein and that a non-opsin-like receptor can couple with a transducin subunit.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 15:44:07