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Titolo:
Hepatitis C virus from the hearts of patients with myocarditis and cardiomyopathy
Autore:
Matsumori, A; Yutani, C; Ikeda, Y; Kawai, S; Sasayama, S;
Indirizzi:
Kyoto Univ, Grad Sch Med, Dept Cardiovasc Med, Sakyo Ku, Kyoto 6068397, Japan Kyoto Univ Kyoto Japan 6068397 ovasc Med, Sakyo Ku, Kyoto 6068397, Japan Natl Cardiovasc Ctr, Dept Pathol, Osaka, Japan Natl Cardiovasc Ctr OsakaJapan rdiovasc Ctr, Dept Pathol, Osaka, Japan Juntendo Univ, Dept Cardiol, Tokyo, Japan Juntendo Univ Tokyo JapanJuntendo Univ, Dept Cardiol, Tokyo, Japan
Titolo Testata:
LABORATORY INVESTIGATION
fascicolo: 7, volume: 80, anno: 2000,
pagine: 1137 - 1142
SICI:
0023-6837(200007)80:7<1137:HCVFTH>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
LEFT-VENTRICULAR DILATATION; POLYMERASE CHAIN-REACTION; HYPERTROPHIC CARDIOMYOPATHY; DILATED CARDIOMYOPATHY; EMBEDDED TISSUES; NON-A; INFECTION; DIAGNOSIS; DISEASE; AMPLIFICATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Matsumori, A Kyoto Univ, Grad Sch Med, Dept Cardiovasc Med, Sakyo Ku, 54 Kawahara Cho Shogoin, Kyoto 6068397, Japan Kyoto Univ 54 Kawahara Cho Shogoin Kyoto Japan 6068397 Japan
Citazione:
A. Matsumori et al., "Hepatitis C virus from the hearts of patients with myocarditis and cardiomyopathy", LAB INV, 80(7), 2000, pp. 1137-1142

Abstract

The myocardium may be the target of several types of viral infections. Theimportance of hepatitis C virus (HCV) infection has been recently noted inpatients with myocarditis and in patients with dilated or hypertrophic cardiomyopathy. The present study sought to detect HCV genomes in formalin-fixed paraffin sections of autopsied hearts from patients with myocarditis andpatients with dilated or hypertrophic cardiomyopathy. Paraffin sections were deparaffinized, RNA was extracted, and the positive and negative strandsof HCV RNA were detected by performing reverse transcription and nested polymerase chain reaction. The polymerase chain reaction products were clonedand sequenced. beta-actin gene was used as a control for the successful amplification of a housekeeping gene. Among 106 hearts examined, beta-actin gene was amplified in 61 hearts (57.5%). Among the latter, HCV RNA was detected in 13 hearts (21.3%), and negative strands in 4 hearts (6.6%). HCV RNA was found in 4 hearts (33.3%) with myocarditis, in 3 hearts (11.5%) with dilated cardiomyopathy, and in 6 hearts (26.0%) with hypertrophic cardiomyopathy. The sequences recovered from nine patients were highly homologous to the standard strain of HCV. HCV genomes were not found in either 35 hearts from patients with myocardial infarction or 20 hearts from patients with noncardiac diseases. These HCV RNA positive samples were obtained from 1 heartin 1979, 7 hearts between 1980 and 1989, and 5 hearts since 1990, indicating that HCV RNA can be amplified from paraffin-embedded hearts preserved for many years. This method of detecting HCV genomes in formalin-fixed paraffin cardiac specimens has enabled us to widen our research into HCV infection and has been helpful in identifying the presence of HCV infection in cardiac myopathic disorders.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 19:29:12