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Titolo:
Lactated ringer's solution and hetastarch but not plasma resuscitation after rat hemorrhagic shock is associated with immediate lung apoptosis by theup-regulation of the bax protein
Autore:
Subrato, D; Sun, L; Martin, B; Talens, E; Burris, D; Kaufmann, C; Rich, N; Rhee, P;
Indirizzi:
Uniformed Serv Univ Hlth Sci, Dept Surg, Bethesda, MD 20814 USA Uniformed Serv Univ Hlth Sci Bethesda MD USA 20814 Bethesda, MD 20814 USA Natl Naval Med Ctr, Dept Surg, Bethesda, MD 20814 USA Natl Naval Med Ctr Bethesda MD USA 20814 ept Surg, Bethesda, MD 20814 USA Walter Reed Army Med Ctr, Washington, DC 20307 USA Walter Reed Army Med Ctr Washington DC USA 20307 Washington, DC 20307 USA
Titolo Testata:
JOURNAL OF TRAUMA-INJURY INFECTION AND CRITICAL CARE
fascicolo: 1, volume: 49, anno: 2000,
pagine: 47 - 53
Fonte:
ISI
Lingua:
ENG
Soggetto:
ISCHEMIA-REPERFUSION INJURY; CELL-DEATH; TRAUMA-HEMORRHAGE; BCL-2; EXPRESSION; DISEASE; RELEASE; PHASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Rhee, P Uniformed Serv Univ Hlth Sci, Dept Surg, 4301 Jones Bridge Rd, Bethesda, MD 20814 USA Uniformed Serv Univ Hlth Sci 4301 Jones Bridge Rd Bethesda MD USA 20814
Citazione:
D. Subrato et al., "Lactated ringer's solution and hetastarch but not plasma resuscitation after rat hemorrhagic shock is associated with immediate lung apoptosis by theup-regulation of the bax protein", J TRAUMA, 49(1), 2000, pp. 47-53

Abstract

Background: We previously demonstrated that the type of resuscitation fluid used in hemorrhagic shock affects apoptosis. Unlike crystalloid, whole blood seems to attenuate programmed cell death. The purpose of this study wasto determine whether the acellular components of whole blood (plasma, albumin) attenuated apoptosis and to determine whether this process involved the Bar protein pathway. Methods: Rats were hemorrhaged 27.5 mL/kg, kept in hypovolemic shock for 75 minutes, then resuscitated over 1 hour (n = 44). Control animals underwent anesthesia only (sham, n = 7), Treatment animals were bled then randomly assigned to the following resuscitation groups: no resuscitation (n = 6), whole blood (n = 6), plasma (:n = 6), 5% human albumin (n = 6), 6% hetastarch (n = 7), and lactated Ringer's solution (LR, n = 6), Hetastarch was used to control for any colloid effect. LR was used as positive control. Immediately after resuscitation, the lung was collected and evaluated for apoptosis by using two methods. TUNEL stain was used to determine general DNA damage, and Bar protein was used to specifically determine intrinsic pathway involvement. Results: LR and hetastarch treatment resulted in significantly increased apoptosis in the lung as determined by both TUNEL and Bar expression (p < 0.05). Plasma infusion resulted in significantly less apoptosis than LR and hetastarch resuscitation, Multiple cell types (epithelium, endothelium, smooth muscle, monocytes) underwent apoptosis in the lung as demonstrated by the TUNEL stain, whereas Bar expression was limited to cells residing in the perivascular and peribronchial spaces. Conclusion: Apoptosis after volume resuscitation of hemorrhagic shock can be affected by the type of resuscitation fluid used. Manufactured fluids such as lactated Ringer's solution and 6% hetastarch resuscitation resulted in the highest degree of lung apoptosis, The plasma component of whole bloodresulted in the least apoptosis, The process of apoptosis after hemorrhagic shock resuscitation involves the Bar protein.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 07:40:29