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Titolo:
Analysis of B cell epitopes of a glycoprotein porcine zona pellucida (pZP1)
Autore:
Shigeta, M; Hasegawa, A; Hamada, Y; Koyama, K;
Indirizzi:
Hyogo Coll Med, Inst Adv Med Sci, Lab Dev Biol & Reprod, Nishinomiya, Hyogo 663, Japan Hyogo Coll Med Nishinomiya Hyogo Japan 663 Nishinomiya, Hyogo 663, Japan Hyogo Med Univ, Dept Obstet & Gynecol, Nishinomiya, Hyogo 6638501, Japan Hyogo Med Univ Nishinomiya Hyogo Japan 6638501 miya, Hyogo 6638501, Japan
Titolo Testata:
JOURNAL OF REPRODUCTIVE IMMUNOLOGY
fascicolo: 2, volume: 47, anno: 2000,
pagine: 159 - 168
SICI:
0165-0378(200007)47:2<159:AOBCEO>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
MONOCLONAL-ANTIBODIES; CONTRACEPTIVE VACCINES; ACTIVE IMMUNIZATION; ANTIGENS; FAMILY; INHIBITION; FERTILITY; MICE; ZP4; GONADOTROPIN;
Keywords:
porcine zona pellucida; immunocontraceptive vaccine; B-cell epitope; in vitro fertilization;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Koyama, K Hyogo Coll Med, Inst Adv Med Sci, Lab Dev Biol & Reprod, Nishinomiya, Hyogo 663, Japan Hyogo Coll Med Nishinomiya Hyogo Japan 663 ya, Hyogo 663, Japan
Citazione:
M. Shigeta et al., "Analysis of B cell epitopes of a glycoprotein porcine zona pellucida (pZP1)", J REPRO IMM, 47(2), 2000, pp. 159-168

Abstract

The zona pellucida (ZP) of mammalian oocytes forms an extracellular matrixcomposed of three major glycoproteins and plays an important role in sperm-zona interactions. As ZP had a strong organ-specific but species-cross-reactive antigenicity and passive or active immunization with ZP antigens could impair fertilization, the possibility of developing a immunocontraceptivevaccine has been extensively studied. Studies on active immunization with porcine ZP (pZP) that contain B cell epitopes and T cell epitopes demonstrated that a temporary infertility could be induced along with the elevation of antibody titers, but it was always associated with ovarian failure. Thiscould be due to the oophoritis by activation of pathogenic T cell immunity. It is the general consideration that any adverse effects by vaccination should be avoided for an immunocontraception. From this point of view, the analysis of B cell epitopes of pZP protein would be helpful for constructionof a safe immunocontraceptive vaccine with zona antigens. We determined the amino acid sequence of the B epitope in the pZPI protein by using a monoclonal antibody (MAb-5H4) that possesses a fertilization blocking ability. In addition, antiserum raised to the epitope sequence was revealed to block in vitro fertilization of homologous animal species. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/11/20 alle ore 14:28:42