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Titolo:
Oestradiol regulation of oxytocin receptor expression in cyclic bovine endometrium
Autore:
Leung, ST; Wathes, DC;
Indirizzi:
Univ London Royal Vet Coll, Dept Vet Basic Sci, Potters Bar EN6 1NB, Herts, England Univ London Royal Vet Coll Potters Bar Herts England EN6 1NB rts, England
Titolo Testata:
JOURNAL OF REPRODUCTION AND FERTILITY
fascicolo: 2, volume: 119, anno: 2000,
pagine: 287 - 292
SICI:
0022-4251(200007)119:2<287:OROORE>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
UTERINE OXYTOCIN; EARLY-PREGNANCY; ESTROUS-CYCLE; PROGESTERONE-RECEPTOR; OVARIECTOMIZED EWES; OVINE ENDOMETRIUM; ESTRADIOL; UTERUS; LOCALIZATION; INTERFERONS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
26
Recensione:
Indirizzi per estratti:
Indirizzo: Wathes, DC Univ London Royal Vet Coll, Dept Vet Basic Sci, Boltons Pk,Hawkshead Rd, Potters Bar EN6 1NB, Herts, England Univ London Royal Vet Coll Boltons Pk,Hawkshead Rd Potters Bar Herts England EN6 1NB
Citazione:
S.T. Leung e D.C. Wathes, "Oestradiol regulation of oxytocin receptor expression in cyclic bovine endometrium", J REPR FERT, 119(2), 2000, pp. 287-292

Abstract

Oestradiol treatment can increase uterine oxytocin receptor expression in vivo. The actions of oestrogen are usually mediated via its receptor, but it also has direct nongenomic effects in some cells. This study investigatedthe effect of oestradiol and the role of the oestradiol receptor in regulating endometrial oxytocin receptor expression in the bovine uterus. Explantcultures (in triplicate) from late luteal phase non-pregnant endometrium received the following treatments: control (serum-free medium), oestradiol (0.1 and 0.01 mu mol l(-1)), oestradiol (0.1 mu mol l(-1)) with the oestradiol receptor antagonist ICI 182780 (0.5 mu mol l(-1)), and ICI 182780 (0.5 mu mol l(-1)) alone. Explants were collected 12, 24 and 48 h after the startof culture. Oxytocin receptor mRNA expression in the explants was measuredby in situ hybridization and oxytocin protein concentrations were measuredby autoradiography with the iodinated oxytocin receptor antagonist d(CH2)(5) [Tyr (Me)(2) Thr(4) Tyr NH29]-vasotocin (I-125-labelled oxytocin receptor antagonist). Oxytocin receptor mRNA and protein expression were initiallylow but spontaneous upregulation occurred in the luminal epithelium between 24 and 48 h (P < 0.01). Oestradiol increased oxytocin receptor mRNA upregulation in the first 24 h (P < 0.05) but the effect on 125I-labelled oxytocin receptor antagonist binding was not significant. ICI 182780 inhibited the oestrogenic effect but had no significant effect on oxytocin receptor mRNA expression when given alone. Ln conclusion, the results showed that oestradiol exerts its effect via the oestradiol receptor. Oestradiol facilitatesoxytocin receptor gene transcription by increasing it more rapidly, but spontaneous upregulation of endometrial oxytocin receptor still occurs in theabsence of oestradiol.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/09/20 alle ore 01:28:20