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Titolo:
Effects of different activation treatments on fertilization of horse oocytes by intracytoplasmic sperm injection
Autore:
Li, XH; Morris, LHA; Allen, WR;
Indirizzi:
Univ Cambridge, Dept Vet Clin Med, Equine Fertil Unit, Newmarket CB8 9BH, Suffolk, England Univ Cambridge Newmarket Suffolk England CB8 9BH B8 9BH, Suffolk, England
Titolo Testata:
JOURNAL OF REPRODUCTION AND FERTILITY
fascicolo: 2, volume: 119, anno: 2000,
pagine: 253 - 260
SICI:
0022-4251(200007)119:2<253:EODATO>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
INDUCED CALCIUM-RELEASE; BOVINE OOCYTES; INTRACELLULAR CALCIUM; RABBIT EGGS; PARTHENOGENETIC DEVELOPMENT; INOSITOL TRISPHOSPHATE; PORCINE OOCYTES; MOUSE OOCYTES; HAMSTER EGGS; THIMEROSAL;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
48
Recensione:
Indirizzi per estratti:
Indirizzo: Allen, WR Univ Cambridge, Dept Vet Clin Med, Equine Fertil Unit, Mertoun Paddocks,Woodditton Rd, Newmarket CB8 9BH, Suffolk, England Univ Cambridge Mertoun Paddocks,Woodditton Rd Newmarket Suffolk England CB8 9BH
Citazione:
X.H. Li et al., "Effects of different activation treatments on fertilization of horse oocytes by intracytoplasmic sperm injection", J REPR FERT, 119(2), 2000, pp. 253-260

Abstract

The effects of four reagents on the activation and subsequent fertilization of equine oocytes, and the development of these after intracytoplasmic sperm injection, were investigated. Cumulus-oocyte complexes collected from equine ovaries obtained from an abattoir were matured in vitro for 40-44 h in TCM199 medium before being injected, when in metaphase II, with an immobilized stallion spermatozoon. The cumulus-oocyte complexes were then subjected to one of five activation treatments: (a) 10 mu mol ionomycin l(-1) for 10 min; Co) 7% (v/v) ethanol for 10 min; (c) 100 mu mol thimerosal l(-1) for 10 min; (d) 250 mu mol inositol 1,4,5-triphosphate l(-1) injection; and (e) no treatment (control). After 18-20 h further culture, the cumulus-oocyte complexes were assessed for activation by observing whether they had progressed through second anaphase-telophase and had formed a female pronucleus. The proportions of oocytes activated after each treatment were: 16/27 (59%) for ionomycin; 14/25 (56%) for ethanol; 22/28 (79%) for thimerosal; 15/27 (56%) for inositol 1,4,5-triphosphate; and 0/20 (0%) for the untreated controls. Thus, significantly more oocytes (P < 0.05) were activated by treatment with thimerosal than by the other four treatments. The proportions of oocytes that cleaved to the two-cell stage at 24-30 h after sperm injectionin the groups treated with ionomycin, ethanol and thimerosal were 7/20 (35%), 5/19 (26%) and 11/23 (48%), respectively. No cleavage was observed in any of the control oocytes or those treated with inositol 1,4,5-triphosphate, Furthermore, evidence of normal fertilization was observed in 2/7 (29%), 2/5 (40%) and 7/11 (64%) of the oocytes treated with ionomycin, ethanol andthimerosal, respectively. These results demonstrated that: (a) it is possible to activate equine oocytes with the chemical stimulants, ionomycin, ethanol, thimerosal and inositol 1,4,5-triphosphate; Cb) thimerosal is more effective than the other three reagents in facilitating both meiotic activation and normal fertilization of equine oocytes; and (c) chemical activation may also stimulate parthenogenetic cleavage of oocytes without concurrent changes in the head of the spermatozoon.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/10/20 alle ore 11:27:01