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Titolo:
A novel, 11 nucleotide variant of chi, chi*: One of a class of sequences defining the Escherichia coli recombination hotspot chi
Autore:
Arnold, DA; Handa, N; Kobayashi, I; Kowalczykowski, SC;
Indirizzi:
Univ Calif Davis, Microbiol Sect, Davis, CA 95616 USA Univ Calif Davis Davis CA USA 95616 , Microbiol Sect, Davis, CA 95616 USA Univ Calif Davis, Sect Mol & Cellular Biol, Davis, CA 95616 USA Univ CalifDavis Davis CA USA 95616 & Cellular Biol, Davis, CA 95616 USA Univ Calif Davis, Genet Grad Grp, Davis, CA 95616 USA Univ Calif Davis Davis CA USA 95616 , Genet Grad Grp, Davis, CA 95616 USA Univ Tokyo, Inst Med Sci, Dept Mol Biol, Minato Ku, Tokyo 1088639, Japan Univ Tokyo Tokyo Japan 1088639 Mol Biol, Minato Ku, Tokyo 1088639, Japan
Titolo Testata:
JOURNAL OF MOLECULAR BIOLOGY
fascicolo: 3, volume: 300, anno: 2000,
pagine: 469 - 479
SICI:
0022-2836(20000714)300:3<469:AN1NVO>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
HOT SPOT ACTIVITY; RECBCD ENZYME; HOMOLOGOUS RECOMBINATION; MEDIATED RECOMBINATION; BACTERIOPHAGE-LAMBDA; PHAGE-LAMBDA; SITE; ORIENTATION; MUTATIONS; RECA;
Keywords:
RecBCD enzyme; chi (chi); RecA protein; genetic recombination; recombination hotspot;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Kowalczykowski, SC Univ Calif Davis, Microbiol Sect, Davis, CA 95616 USA Univ Calif Davis Davis CA USA 95616 avis, CA 95616 USA
Citazione:
D.A. Arnold et al., "A novel, 11 nucleotide variant of chi, chi*: One of a class of sequences defining the Escherichia coli recombination hotspot chi", J MOL BIOL, 300(3), 2000, pp. 469-479

Abstract

In wild-type Escherichia coil, recognition of the recombination hotspot, A(5'-GCTGGTGG-3'), by the RecBCD enzyme is central to homologous recombination. However, in the recC* class of RecBCD mutants, stimulation of recombination by the canonical chi sequence is not detectable, but the levels of homologous recombination are nearly wild-type. In vivo studies demonstrate that a member of this class of mutants, the recC1004 allele, encodes an enzymethat responds to a novel variant of chi, termed chi* (5'-GCTGGTGCTCG-3'). Here, we establish that, in vitro, the chi* sequence is recognized more efficiently by the RecBC(1004)D enzyme than is the wild-type chi. This is manifest by both a greater modification of nuclease activity and a higher stimulation of RecA protein-mediated joint molecule formation at chi* than at chi Sequencing of the recC1004 gene revealed that it contains a frameshift mutation, which results in a replacement of nine of the wild-type amino acid residues by eight in the mutant protein, and defines a locus that is important for the specificity of chi-recognition. Ln addition, we show that this novel, 11 nucleotide chi* sequence also regulates the wild-type RecBCD enzyme, supporting the notion that variants of the canonical chi, constitute a class of sequences that regulate the recombination function of RecBCD enzyme. (C) 2000 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/04/20 alle ore 11:05:03