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Titolo:
Relative importance of the LDL receptor and scavenger receptor class B in the beta-VLDL-induced uptake and accumulation of cholesteryl esters by peritoneal macrophages
Autore:
Herijgers, N; Van Eck, M; Korporaal, SJA; Hoogerbrugge, PM; Van Berkel, TJC;
Indirizzi:
Leiden Univ, Sylvius Labs, Leiden Amsterdam Ctr Drug Res, Div Biopharmaceut, NL-2333 AL Leiden, Netherlands Leiden Univ Leiden Netherlands NL-2333 AL NL-2333 AL Leiden, Netherlands Univ Nijmegen Hosp, Dept Pediat, NL-6525 GA Nijmegen, Netherlands Univ Nijmegen Hosp Nijmegen Netherlands NL-6525 GA Nijmegen, Netherlands
Titolo Testata:
JOURNAL OF LIPID RESEARCH
fascicolo: 7, volume: 41, anno: 2000,
pagine: 1163 - 1171
SICI:
0022-2275(200007)41:7<1163:RIOTLR>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
LOW-DENSITY-LIPOPROTEIN; BONE-MARROW TRANSPLANTATION; HUMAN MONOCYTE-MACROPHAGES; PARENCHYMAL LIVER-CELLS; SELECTIVE UPTAKE; SR-BI; APOLIPOPROTEIN-E; ALPHA-2-MACROGLOBULIN RECEPTOR; CHYLOMICRON REMNANTS; MEDIATED UPTAKE;
Keywords:
atherosclerosis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
62
Recensione:
Indirizzi per estratti:
Indirizzo: Van Berkel, TJC Leiden Univ, Sylvius Labs, Leiden Amsterdam Ctr Drug Res, Div Biopharmaceut, NL-2333 AL Leiden, Netherlands Leiden Univ Leiden Netherlands NL-2333 AL n, Netherlands
Citazione:
N. Herijgers et al., "Relative importance of the LDL receptor and scavenger receptor class B in the beta-VLDL-induced uptake and accumulation of cholesteryl esters by peritoneal macrophages", J LIPID RES, 41(7), 2000, pp. 1163-1171

Abstract

We investigated the mechanism of beta-very low density lipoprotein (beta-VLDL)-induced foam cell formation derived from peritoneal macrophages from control mice and low density lipoprotein (LDL) receptor-deficient mice to elucidate the role of the LDL receptor in this process. The LDL receptor appeared to be of major importance for beta-VLDL metabolism. Consequently, the accumulation of cholesteryl esters in LDL receptor(-/-) macrophages is 2.5-fold lower than in LDL receptor(+/+) macrophages, In the absence of the LDLreceptor, however, beta-VLDL was still able to induce cholesteryl ester accumulation and subsequently we characterized the properties of this residual beta-VLDL recognition site(s) of LDL receptor(-/-) macrophages, Although the LDL receptor-related protein is expressed on LDL receptor(-/-) macrophages, the cell association of beta-VLDL is not influenced by the receptor-associated protein, and treatment of the macrophages with heparinase and chondroitinase was also ineffective. In contrast, both oxidized LDL (OxLDL) andanionic liposomes were able to inhibit the cell association of I-125-labeled beta-VLDL in LDL receptor(-/-) macrophages by 65%. These properties suggest a role for scavenger receptor class B (SR-B), and indeed, in the LDL receptor(-/-) macrophages the selective uptake of cholesteryl esters from beta-VLDL was 2.2-fold higher than that of apolipoproteins, a process that could be inhibited by OxLDL, high density lipoprotein (HDL), and beta-VLDL. Inconclusion, the LDL receptor on peritoneal macrophages is directly involved in the metabolism of beta-VLDL and the subsequent foam cell formation. When the LDL receptor is absent, SR-B appears to mediate the remaining metabolism of cholesteryl esters from beta-VLDL.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/02/20 alle ore 08:35:55