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Titolo:
Increased uptake of alpha-hydroxy aldehyde-modified low density lipoprotein by macrophage scavenger receptors
Autore:
Kawamura, M; Heinecke, JW; Chait, A;
Indirizzi:
Univ Washington, Dept Med, Seattle, WA 98195 USA Univ Washington Seattle WA USA 98195 ton, Dept Med, Seattle, WA 98195 USA Washington Univ, Sch Med, Dept Lipid Res, St Louis, MO 63110 USA Washington Univ St Louis MO USA 63110 t Lipid Res, St Louis, MO 63110 USA
Titolo Testata:
JOURNAL OF LIPID RESEARCH
fascicolo: 7, volume: 41, anno: 2000,
pagine: 1054 - 1059
SICI:
0022-2275(200007)41:7<1054:IUOAAL>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
SMOOTH-MUSCLE CELLS; HUMAN MONOCYTE-MACROPHAGES; AMINO-ACIDS; ALPHA,BETA-UNSATURATED ALDEHYDES; HUMAN PHAGOCYTES; IN-VIVO; OXIDATION; MYELOPEROXIDASE; METABOLISM; MECHANISM;
Keywords:
lipids; oxidation; aldehydes; macrophages; atherosclerosis; diabetes;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Chait, A Univ Washington, Dept Med, Seattle, WA 98195 USA Univ WashingtonSeattle WA USA 98195 Med, Seattle, WA 98195 USA
Citazione:
M. Kawamura et al., "Increased uptake of alpha-hydroxy aldehyde-modified low density lipoprotein by macrophage scavenger receptors", J LIPID RES, 41(7), 2000, pp. 1054-1059

Abstract

Reactive aldehydes can be formed during the oxidation of lipids, glucose, and amino acids and during the nonenzymatic glycation of proteins. Low density lipoprotein (LDL) modified with malondialdehyde are taken up by scavenger receptors on macrophages, In the current studies we determined whether alpha-hydroxy aldehydes also modify LDL to a form recognized by macrophage scavenger receptors, LDL modified by incubation with glycolaldehyde, glyceraldehyde, erythrose, arabinose, or glucose (alpha-hydroxy aldehydes that possess two, three, four, five, and six carbon atoms, respectively) exhibited decreased free amino groups and increased mobility on agarose gel electrophoresis. The lower the molecular weight of the aldehyde used for LDL modification, the more rapid and extensive was the derivatization of free amino groups. Approximately 50-75% of free lysine groups in LDL were modified afterincubation with glyceraldehyde, glycolaldehyde, or erythrose for 24-48 h. Less extensive reductions in free amino groups were observed when LDL was incubated with arabinose or glucose, even at high concentration for up to 5 days. LDL modified with glycolaldehyde and glyceraldehyde labeled with I-125 was degraded more extensively by human monocyte-derived macrophages than was I-125-labeled native LDL. Conversely, LDL modified with I-125-labeled erythrose, arabinose, or glucose was degraded less rapidly than I-125-labeled native LDL. Competition for the degradation of LDL modified with I-125- labeled glyceraldehyde was nearly complete with acetyl-, glycolaldehyde-, and glyceraldehyde-modified LDL, fucoidin, and advanced glycation end product-modified bovine serum albumin, and absent with unlabeled native LDL. Theseresults suggest that short-chain alpha-hydroxy aldehydes react with amino groups on LDL to yield moieties that are important determinants of recognition by macrophage scavenger receptors.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/02/20 alle ore 11:27:14